Important Parameters for Evaluation of Antibody Avidity by Immunosorbent Assay

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 2011 Aug 2

PMID 21803020

Citations 30

Authors

Jordan D Dimitrov

Sebastien Lacroix-Desmazes

Srinivas V Kaveri

Affiliations

Soon will be listed here.

Abstract

The evaluation of antibody avidity by elution with chaotropic agents is a frequently used approach in research and diagnostics. It provides important information on the functional relevance of antibodies. However, in the literature, there is a large heterogeneity in the experimental settings for the determination of this important parameter. Here, we demonstrate that antibody concentration and the choice of chaotropic agent are critical for the reliable estimation of antibody avidity.

Citing Articles

Antibody Avidity Maturation Following Booster Vaccination with an Intranasal Adenovirus Salnavac Vaccine.

Astakhova E, Baranov K, Shilova N, Polyakova S, Zuev E, Poteryaev D Vaccines (Basel). 2025; 12(12.

PMID: 39772024 PMC: 11680177. DOI: 10.3390/vaccines12121362.

Sequence Matters: Primary COVID-19 Vaccination after Infection Elicits Similar Anti-spike Antibody Levels, but Stronger Antibody Dependent Cell-mediated Cytotoxicity than Breakthrough Infection.

Holder K, Ings D, Fifield K, Barnes D, Barnable K, Harnum D J Immunol. 2024; 213(8):1105-1114.

PMID: 39248629 PMC: 11457723. DOI: 10.4049/jimmunol.2400250.

An unexpected IgE anti-receptor binding domain response following natural infection and different types of SARS-CoV-2 vaccines.

Portilho A, Silva V, Hermes Monteiro da Costa H, Yamashiro R, de Oliveira I, de Campos I Sci Rep. 2024; 14(1):20003.

PMID: 39198569 PMC: 11358332. DOI: 10.1038/s41598-024-71047-5.

Avidity maturation of humoral response following primary and booster doses of BNT162b2 mRNA vaccine among nursing home residents and healthcare workers.

Oyebanji O, Sundheimer N, Ragavapuram V, Wilson B, Abul Y, Gravenstein S Geroscience. 2024; 46(6):6183-6194.

PMID: 38789833 PMC: 11493945. DOI: 10.1007/s11357-024-01215-y.

Hybrid response to SARS-CoV-2 and C after an OMV-adjuvanted immunization in mice and their offspring.

Portilho A, Hermes Monteiro da Costa H, Grando Guereschi M, Prudencio C, De Gaspari E Hum Vaccin Immunother. 2024; 20(1):2346963.

PMID: 38745461 PMC: 11789737. DOI: 10.1080/21645515.2024.2346963.