Amplified Detection of Cocaine Based on Strand-displacement Polymerization and Fluorescence Resonance Energy Transfer

Overview

Journal Biosens Bioelectron

Specialties Biochemistry
Biotechnology

Date 2011 Aug 2

PMID 21802274

Citations 3

Authors

Jin Huang

Yan Chen

Liu Yang

Zhi Zhu

Guizhi Zhu

Xiaohai Yang

Kemin Wang

Weihong Tan

Affiliations

Soon will be listed here.

Abstract

Cocaine is one of the most abused drugs in the United States and is potentially dangerous when consumed in excess. Its detection is thus important in many areas in the fight against drug trafficking. We have developed an amplified detection method for cocaine based on a strand-displacement polymerization reaction using aptamer recognition. The system mainly consists of a hairpin probe with Cy5 labeled on its 3' end, a primer with FAM labeled on its 5' end, and polymerase. The aptamer sequence is integrated into the 5'-section of the hairpin probe. The primer is designed complementary to the 3' end of the hairpin probe, which is also part of the hairpin stem region. The cocaine induced reaction cycle generates product for detection and thus for signal amplification. The detection limit of this method is 200 nM in about 16 min and the specificity of this approach is excellent. We believe that this strategy will be useful for the development of analytical schemes for a variety of aptamers for small molecules, metal ions, and proteins. This simple scheme employing the strand-displacement polymerization reaction may find wide application in forensic analysis, environmental monitoring, and clinical diagnostics.

Citing Articles

Nanomaterial-based aptamer sensors for analysis of illicit drugs and evaluation of drugs consumption for wastewater-based epidemiology.

Mao K, Zhang H, Pan Y, Zhang K, Cao H, Li X Trends Analyt Chem. 2020; 130:115975.

PMID: 32834242 PMC: 7336936. DOI: 10.1016/j.trac.2020.115975.

Rapid detection of cocaine using aptamer-based biosensor on an evanescent wave fibre platform.

Qiu Y, Tang Y, Li B, He M R Soc Open Sci. 2018; 5(10):180821.

PMID: 30473831 PMC: 6227954. DOI: 10.1098/rsos.180821.

Chromogenic and Fluorogenic Probes for the Detection of Illicit Drugs.

Garrido E, Pla L, Lozano-Torres B, El Sayed S, Martinez-Manez R, Sancenon F ChemistryOpen. 2018; 7(5):401-428.

PMID: 29872615 PMC: 5974560. DOI: 10.1002/open.201800034.

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