Large-scale Production and Purification of VLP-based Vaccines

Overview

Journal J Invertebr Pathol

Specialty Biology

Date 2011 Jul 26

PMID 21784230

Citations 86

Authors

Tiago Vicente

Antonio Roldao

Cristina Peixoto

Manuel J T Carrondo

Paula M Alves

Affiliations

Soon will be listed here.

Abstract

Virus-like particles (VLPs) hold tremendous potential as vaccine candidates. These innovative biopharmaceuticals present the remarkable advantages of closely mimicking the three-dimensional nature of an actual virus while lacking the virus genome packaged inside its capsid. As a result, an equally efficient but safer prophylaxis is anticipated as compared to inactivated or live attenuated viral vaccines. With the advent of successful cases of approved VLP-based vaccines, pharmaceutical companies are indeed redirecting their resources to the development of such products. This paper reviews the current choices and trends of large-scale production and purification of VLP-based vaccines generated through the baculovirus expression vector system using insect cells.

Citing Articles

Recent Advances in Antiviral Drug Delivery Strategies.

Rana D, Prajapati A, Karunakaran B, Vora L, Benival D, Jindal A AAPS PharmSciTech. 2025; 26(3):73.

PMID: 40038154 DOI: 10.1208/s12249-025-03053-3.

Influenza Neuraminidase Virus-Like Particle-Based Nanocarriers as a New Platform for the Delivery of Small-Peptide Antigens.

Khanefard N, Trianti I, Akeprathumchai S, Mekvichitsaeng P, Roshorm Y, Poomputsa K Mol Biotechnol. 2025; .

PMID: 40021575 DOI: 10.1007/s12033-025-01403-x.

Dimming the corona: studying SARS-coronavirus-2 at reduced biocontainment level using replicons and virus-like particles.

Neilsen G, Mathew A, Castro J, McFadden W, Wen X, Ong Y mBio. 2024; 15(12):e0336823.

PMID: 39530689 PMC: 11633226. DOI: 10.1128/mbio.03368-23.

Virus-like particles in poultry disease: an approach to effective and safe vaccination.

Raji A, Dastjerdi P, Omar A Front Vet Sci. 2024; 11:1405605.

PMID: 39315089 PMC: 11417104. DOI: 10.3389/fvets.2024.1405605.

Virus-like Particles as Vaccines for Allergen-Specific Therapy: An Overview of Current Developments.

Berreiros-Hortala H, Vilchez-Pinto G, Diaz-Perales A, Garrido-Arandia M, Tome-Amat J Int J Mol Sci. 2024; 25(13).

PMID: 39000536 PMC: 11242184. DOI: 10.3390/ijms25137429.

References

Betenbaugh M, Yu M, Kuehl K, White J, Pennock D, Spik K . Nucleocapsid- and virus-like particles assemble in cells infected with recombinant baculoviruses or vaccinia viruses expressing the M and the S segments of Hantaan virus. Virus Res. 1995; 38(2-3):111-24. DOI: 10.1016/0168-1702(95)00053-s. View

Wu C, Yeh Y, Yang Y, Chou C, Liu M, Wu H . Mammalian expression of virus-like particles for advanced mimicry of authentic influenza virus. PLoS One. 2010; 5(3):e9784. PMC: 2842297. DOI: 10.1371/journal.pone.0009784. View

Wolff M, Siewert C, Hansen S, Faber R, Reichl U . Purification of cell culture-derived modified vaccinia ankara virus by pseudo-affinity membrane adsorbers and hydrophobic interaction chromatography. Biotechnol Bioeng. 2010; 107(2):312-20. DOI: 10.1002/bit.22797. View

Peixoto C, Sousa M, Silva A, Carrondo M, Alves P . Downstream processing of triple layered rotavirus like particles. J Biotechnol. 2006; 127(3):452-61. DOI: 10.1016/j.jbiotec.2006.08.002. View

Teixeira A, Oliveira R, Alves P, Carrondo M . Advances in on-line monitoring and control of mammalian cell cultures: Supporting the PAT initiative. Biotechnol Adv. 2009; 27(6):726-732. DOI: 10.1016/j.biotechadv.2009.05.003. View

-hang G, Chen C, Lin C, Chen H, Chen H . Improvement of glycosylation in insect cells with mammalian glycosyltransferases. J Biotechnol. 2003; 102(1):61-71. DOI: 10.1016/s0168-1656(02)00364-4. View

Bachmann A, Corpuz G, Hareld W, Wang G, Coller B . A simple method for the rapid purification of copia virus-like particles from Drosophila Schneider 2 cells. J Virol Methods. 2003; 115(2):159-65. DOI: 10.1016/j.jviromet.2003.09.025. View

Gotoh T, Miyazaki Y, Kikuchi K, Bentley W . Investigation of sequential behavior of carboxyl protease and cysteine protease activities in virus-infected Sf-9 insect cell culture by inhibition assay. Appl Microbiol Biotechnol. 2001; 56(5-6):742-9. DOI: 10.1007/s002530100602. View

Morenweiser R . Downstream processing of viral vectors and vaccines. Gene Ther. 2005; 12 Suppl 1:S103-10. DOI: 10.1038/sj.gt.3302624. View

10.

French T, Marshall J, Roy P . Assembly of double-shelled, viruslike particles of bluetongue virus by the simultaneous expression of four structural proteins. J Virol. 1990; 64(12):5695-700. PMC: 248707. DOI: 10.1128/JVI.64.12.5695-5700.1990. View

11.

Shrestha M, Scott R, Joshi D, Mammen Jr M, Thapa G, Thapa N . Safety and efficacy of a recombinant hepatitis E vaccine. N Engl J Med. 2007; 356(9):895-903. DOI: 10.1056/NEJMoa061847. View

12.

Vieira H, Estevao C, Roldao A, Peixoto C, Sousa M, Cruz P . Triple layered rotavirus VLP production: kinetics of vector replication, mRNA stability and recombinant protein production. J Biotechnol. 2005; 120(1):72-82. DOI: 10.1016/j.jbiotec.2005.03.026. View

13.

Saha K, Lin Y, Wong P . A simple method for obtaining highly viable virus from culture supernatant. J Virol Methods. 1994; 46(3):349-52. DOI: 10.1016/0166-0934(94)90005-1. View

14.

Pillay S, Meyers A, Williamson A, Rybicki E . Optimization of chimeric HIV-1 virus-like particle production in a baculovirus-insect cell expression system. Biotechnol Prog. 2009; 25(4):1153-60. DOI: 10.1002/btpr.187. View

15.

Jiang B, Barniak V, Smith R, Sharma R, Corsaro B, Hu B . Synthesis of rotavirus-like particles in insect cells: comparative and quantitative analysis. Biotechnol Bioeng. 1999; 60(3):369-74. DOI: 10.1002/(sici)1097-0290(19981105)60:3<369::aid-bit14>3.0.co;2-h. View

16.

Maranga L, Rueda P, Antonis A, Vela C, Langeveld J, Casal J . Large scale production and downstream processing of a recombinant porcine parvovirus vaccine. Appl Microbiol Biotechnol. 2002; 59(1):45-50. DOI: 10.1007/s00253-002-0976-x. View

17.

Vicente T, Mota J, Peixoto C, Alves P, Carrondo M . Analysis of adsorption of a baculovirus bioreaction bulk on an ion-exchange surface by surface plasmon resonance. J Biotechnol. 2010; 148(4):171-81. DOI: 10.1016/j.jbiotec.2010.05.005. View

18.

Tatman J, Preston V, Nicholson P, Elliott R, Rixon F . Assembly of herpes simplex virus type 1 capsids using a panel of recombinant baculoviruses. J Gen Virol. 1994; 75 ( Pt 5):1101-13. DOI: 10.1099/0022-1317-75-5-1101. View

19.

Mellado M, Mena J, Lopes A, Ramirez O, Carrondo M, Palomares L . Impact of physicochemical parameters on in vitro assembly and disassembly kinetics of recombinant triple-layered rotavirus-like particles. Biotechnol Bioeng. 2009; 104(4):674-86. DOI: 10.1002/bit.22430. View

20.

Wu C, Soh K, Wang S . Ion-exchange membrane chromatography method for rapid and efficient purification of recombinant baculovirus and baculovirus gp64 protein. Hum Gene Ther. 2007; 18(7):665-72. DOI: 10.1089/hum.2007.020. View