Expression of the Ribonucleases Drosha, Dicer, and Ago2 in Colorectal Carcinomas

Overview

Journal Virchows Arch

Specialties Cell Biology
Molecular Biology
Pathology

Date 2011 Jul 20

PMID 21769619

Citations 45

Authors

Dionysios J Papachristou

Angeliki Korpetinou

Efstathia Giannopoulou

Anna G Antonacopoulou

Helen Papadaki

Petros Grivas

Chrisoula D Scopa

Haralabos P Kalofonos

Affiliations

Soon will be listed here.

Abstract

The pathogenesis of colorectal carcinoma (CRC) is a complex process that involves the recruitment of both genetic and epigenetic mechanisms. Recent studies underline the cardinal role of small, noncoding RNA molecules, called microRNAs (miRs), in the pathobiology of numerous physiological and pathological processes, including oncogenesis. MiR biogenesis and maturation is mainly regulated by the nuclear ribonuclease Drosha and the cytoplasmic ribonucleases Dicer and Ago2. In the present study, we investigated the expression and distribution of these molecules in three colon cancer cell lines and in human CRC samples. Drosha, Dicer, and Ago2 mRNA and protein expression was assessed with real-time PCR, western blotting, and immunofluorescence. Our experiments showed that Drosha, Dicer, and Ago2 were expressed in all the cell lines and in the majority of the CRC samples examined. The mRNA levels of Dicer were significantly augmented in stage III compared to stage II tumors. Our results suggest that Drosha, Dicer, and Ago2 are possibly implicated in CRC pathobiology and that Dicer might have a role in the progression of these tumors to advanced stages.

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