Fluorescent Analysis of the Cell-selective Alzheimer's Disease Aβ Peptide Surface Membrane Binding: Influence of Membrane Components

Overview

Journal Int J Alzheimers Dis

Publisher Wiley

Date 2011 Jul 16

PMID 21760991

Citations 4

Authors

Olga Simakova

Nelson J Arispe

Affiliations

Soon will be listed here.

Abstract

We performed a fluorescent analysis of the binding of Aβ to the surface membrane of different types of cells lines such as PC12, GT1-7, and ex vivo neurons. Analyses were performed on sorted cells with membrane bound Aβ Competitive binding between Aβ phosphatidyl serine- (PtdSer-) specific binder annexin V and an anti-PtdSer antibody provided compelling data confirming the involvement of PtdSer as one of the surface membrane signal molecules for Aβ. We found that populations of cells that exhibited high surface membrane binding affinity for Aβ also show higher membrane cholesterol levels compared to cells that did not bind Aβ. This direct relationship was upheld in cholesterol-enriched or cholesterol-depleted cell membranes. We conclude that the initial process for the cell-selective binding by Aβ, to later conversion of elemental Aβ units into larger structures such as fibrils or to the potentially toxic ion channel aggregates, is highly influenced by the membrane content of PtdSer and cholesterol in the cell surface membrane.

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References

Simakova O, Arispe N . Early and late cytotoxic effects of external application of the Alzheimer's Abeta result from the initial formation and function of Abeta ion channels. Biochemistry. 2006; 45(18):5907-15. DOI: 10.1021/bi060148g. View

Yanagisawa K . GM1 ganglioside and the seeding of amyloid in Alzheimer's disease: endogenous seed for Alzheimer amyloid. Neuroscientist. 2005; 11(3):250-60. DOI: 10.1177/1073858405275177. View

Yankner B . Mechanisms of neuronal degeneration in Alzheimer's disease. Neuron. 1996; 16(5):921-32. DOI: 10.1016/s0896-6273(00)80115-4. View

Cecchi C, Rosati F, Pensalfini A, Formigli L, Nosi D, Liguri G . Seladin-1/DHCR24 protects neuroblastoma cells against Abeta toxicity by increasing membrane cholesterol content. J Cell Mol Med. 2008; 12(5B):1990-2002. PMC: 4506165. DOI: 10.1111/j.1582-4934.2008.00216.x. View

Buchsteiner A, Hauss T, Dante S, Dencher N . Alzheimer's disease amyloid-beta peptide analogue alters the ps-dynamics of phospholipid membranes. Biochim Biophys Acta. 2010; 1798(10):1969-76. DOI: 10.1016/j.bbamem.2010.06.024. View

Vargas J, Alarcon J, Rojas E . Displacement currents associated with the insertion of Alzheimer disease amyloid beta-peptide into planar bilayer membranes. Biophys J. 2000; 79(2):934-44. PMC: 1300990. DOI: 10.1016/S0006-3495(00)76348-X. View

Fadok V, Voelker D, Campbell P, Cohen J, Bratton D, Henson P . Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J Immunol. 1992; 148(7):2207-16. View

Simons M, Keller P, De Strooper B, Beyreuther K, Dotti C, Simons K . Cholesterol depletion inhibits the generation of beta-amyloid in hippocampal neurons. Proc Natl Acad Sci U S A. 1998; 95(11):6460-4. PMC: 27798. DOI: 10.1073/pnas.95.11.6460. View

Martin O, Pagano R . Transbilayer movement of fluorescent analogs of phosphatidylserine and phosphatidylethanolamine at the plasma membrane of cultured cells. Evidence for a protein-mediated and ATP-dependent process(es). J Biol Chem. 1987; 262(12):5890-8. View

10.

Wong P, Schauerte J, Wisser K, Ding H, Lee E, Steel D . Amyloid-beta membrane binding and permeabilization are distinct processes influenced separately by membrane charge and fluidity. J Mol Biol. 2008; 386(1):81-96. DOI: 10.1016/j.jmb.2008.11.060. View

11.

Surewicz W . The interaction between Alzheimer amyloid beta(1-40) peptide and ganglioside GM1-containing membranes. FEBS Lett. 1997; 402(2-3):95-8. DOI: 10.1016/s0014-5793(96)01504-9. View

12.

Matsuzaki K, Kato K, Yanagisawa K . Abeta polymerization through interaction with membrane gangliosides. Biochim Biophys Acta. 2010; 1801(8):868-77. DOI: 10.1016/j.bbalip.2010.01.008. View

13.

ZWAAL R, Schroit A . Pathophysiologic implications of membrane phospholipid asymmetry in blood cells. Blood. 1997; 89(4):1121-32. View

14.

Yanagisawa K . Role of gangliosides in Alzheimer's disease. Biochim Biophys Acta. 2007; 1768(8):1943-51. DOI: 10.1016/j.bbamem.2007.01.018. View

15.

Kakio A, Nishimoto S, Yanagisawa K, Kozutsumi Y, Matsuzaki K . Cholesterol-dependent formation of GM1 ganglioside-bound amyloid beta-protein, an endogenous seed for Alzheimer amyloid. J Biol Chem. 2001; 276(27):24985-90. DOI: 10.1074/jbc.M100252200. View

16.

Mirzabekov T, Lin M, Yuan W, Marshall P, Carman M, Tomaselli K . Channel formation in planar lipid bilayers by a neurotoxic fragment of the beta-amyloid peptide. Biochem Biophys Res Commun. 1994; 202(2):1142-8. DOI: 10.1006/bbrc.1994.2047. View

17.

Kawahara M, Kuroda Y, Arispe N, Rojas E . Alzheimer's beta-amyloid, human islet amylin, and prion protein fragment evoke intracellular free calcium elevations by a common mechanism in a hypothalamic GnRH neuronal cell line. J Biol Chem. 2000; 275(19):14077-83. DOI: 10.1074/jbc.275.19.14077. View

18.

Fadok V, Bratton D, Rose D, Pearson A, Ezekewitz R, Henson P . A receptor for phosphatidylserine-specific clearance of apoptotic cells. Nature. 2000; 405(6782):85-90. DOI: 10.1038/35011084. View

19.

Ariga T, Kobayashi K, Hasegawa A, Kiso M, Ishida H, Miyatake T . Characterization of high-affinity binding between gangliosides and amyloid beta-protein. Arch Biochem Biophys. 2001; 388(2):225-30. DOI: 10.1006/abbi.2001.2304. View

20.

Kawahara M, Kuroda Y . Intracellular calcium changes in neuronal cells induced by Alzheimer's beta-amyloid protein are blocked by estradiol and cholesterol. Cell Mol Neurobiol. 2001; 21(1):1-13. PMC: 11533833. DOI: 10.1023/a:1007168910582. View