Improved Biocompatibility of Novel Poly(L-lactic Acid)/β-tricalcium Phosphate Scaffolds Prepared by an Organic Solvent-free Method

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2011 Jul 16

PMID 21760732

Citations 3

Authors

Xue-Feng Zhao

Xiao-Dong Li

Yun-qing Kang

Quan Yuan

Affiliations

Soon will be listed here.

Abstract

A porous poly(L-lactic acid)/β-tricalcium phosphate (PLLA/β-TCP) composite scaffold was fabricated using a novel technique comprising powder mixing, compression molding, low-temperature treatment, and particulate leaching without any organic solvent. The effect of this scaffold on osteoblast proliferation and differentiation was evaluated in vitro. The fabricated scaffold had a homogeneously interconnected porous structure with a porosity of 70% and compressive strength of 1.35 MPa. The methylthiazol tetrazolium values and alkaline phosphatase (ALP) activity of osteoblasts seeded on the solvent-free scaffold were significant higher than those of the control. Using real-time PCR, gene expressions of ALP, osteocalcin, and type 1 collagen were shown to be upregulated. As the method does not use any organic solvent, it eliminates problems associated with organic solvent residue and therefore improves the cell compatibility. It has a promising potential for the preparation of porous scaffold for bone tissue engineering.

Citing Articles

Poly (lactic acid)-based biomaterials for orthopaedic regenerative engineering.

Narayanan G, Vernekar V, Kuyinu E, Laurencin C Adv Drug Deliv Rev. 2016; 107:247-276.

PMID: 27125191 PMC: 5482531. DOI: 10.1016/j.addr.2016.04.015.

Collagen/Beta-Tricalcium Phosphate Based Synthetic Bone Grafts via Dehydrothermal Processing.

Sarikaya B, Aydin H Biomed Res Int. 2015; 2015:576532.

PMID: 26504812 PMC: 4609365. DOI: 10.1155/2015/576532.

Synthesis, Characterization, and Osteoblastic Cell Culture of Poly(L-co-D,L-lactide-co-trimethylene carbonate) Scaffolds.

Messias A, Martins K, Cristina Motta A, de Rezende Duek E Int J Biomater. 2014; 2014:501789.

PMID: 25053947 PMC: 4099256. DOI: 10.1155/2014/501789.

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