Advances in Microfluidic PCR for Point-of-care Infectious Disease Diagnostics

Overview

Journal Biotechnol Adv

Specialties Biochemistry
Biotechnology

Date 2011 Jul 12

PMID 21741465

Citations 95

Authors

Seungkyung Park

Yi Zhang

Shin Lin

Tza-Huei Wang

Samuel Yang

Affiliations

Soon will be listed here.

Abstract

Global burdens from existing or emerging infectious diseases emphasize the need for point-of-care (POC) diagnostics to enhance timely recognition and intervention. Molecular approaches based on PCR methods have made significant inroads by improving detection time and accuracy but are still largely hampered by resource-intensive processing in centralized laboratories, thereby precluding their routine bedside- or field-use. Microfluidic technologies have enabled miniaturization of PCR processes onto a chip device with potential benefits including speed, cost, portability, throughput, and automation. In this review, we provide an overview of recent advances in microfluidic PCR technologies and discuss practical issues and perspectives related to implementing them into infectious disease diagnostics.

Citing Articles

Rapid and comprehensive detection of viral antibodies and nucleic acids via an acoustofluidic integrated molecular diagnostics chip: AIMDx.

Qian J, Xia J, Chiang S, Liu J, Li K, Li F Sci Adv. 2025; 11(3):eadt5464.

PMID: 39813350 PMC: 11734728. DOI: 10.1126/sciadv.adt5464.

Advancements of paper-based sensors for antibiotic-resistant bacterial species identification.

Laliwala A, Pant A, Svechkarev D, Sadykov M, Mohs A NPJ Biosens. 2024; 1(1):17.

PMID: 39678719 PMC: 11645268. DOI: 10.1038/s44328-024-00016-9.

Optimizing Microfluidic Channel Design with High-Performance Materials for Safe Neonatal Drug Delivery.

Archana T, Nachammai N, Praveenkumar S Recent Adv Drug Deliv Formul. 2024; 18(4):294-303.

PMID: 39356100 DOI: 10.2174/0126673878292962240718055526.

Biochemically Programmable Isothermal PCR.

Kim M, Ravisankar V, Hassan Y, Ugaz V Adv Sci (Weinh). 2024; 11(41):e2404688.

PMID: 39269276 PMC: 11538674. DOI: 10.1002/advs.202404688.

Mobile Diagnostic Clinics.

Baron R, Haick H ACS Sens. 2024; 9(6):2777-2792.

PMID: 38775426 PMC: 11217950. DOI: 10.1021/acssensors.4c00636.

References

Kohanski M, Dwyer D, Collins J . How antibiotics kill bacteria: from targets to networks. Nat Rev Microbiol. 2010; 8(6):423-35. PMC: 2896384. DOI: 10.1038/nrmicro2333. View

Beer N, Hindson B, Wheeler E, Hall S, Rose K, Kennedy I . On-chip, real-time, single-copy polymerase chain reaction in picoliter droplets. Anal Chem. 2007; 79(22):8471-5. DOI: 10.1021/ac701809w. View

Easley C, Karlinsey J, Bienvenue J, Legendre L, Roper M, Feldman S . A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability. Proc Natl Acad Sci U S A. 2006; 103(51):19272-7. PMC: 1748216. DOI: 10.1073/pnas.0604663103. View

Hataoka Y, Zhang L, Mori Y, Tomita N, Notomi T, Baba Y . Analysis of specific gene by integration of isothermal amplification and electrophoresis on poly(methyl methacrylate) microchips. Anal Chem. 2004; 76(13):3689-93. DOI: 10.1021/ac035032u. View

Issadore D, Humphry K, Brown K, Sandberg L, Weitz D, Westervelt R . Microwave dielectric heating of drops in microfluidic devices. Lab Chip. 2009; 9(12):1701-6. PMC: 2892413. DOI: 10.1039/b822357b. View

Teh S, Lin R, Hung L, Lee A . Droplet microfluidics. Lab Chip. 2008; 8(2):198-220. DOI: 10.1039/b715524g. View

Lee Y, Lien K, Lei H, Lee G . An integrated microfluidic system for rapid diagnosis of dengue virus infection. Biosens Bioelectron. 2009; 25(4):745-52. PMC: 7125828. DOI: 10.1016/j.bios.2009.08.020. View

Sun Y, Kwok Y . Polymeric microfluidic system for DNA analysis. Anal Chim Acta. 2007; 556(1):80-96. DOI: 10.1016/j.aca.2005.09.035. View

Irimia D, Tompkins R, Toner M . Single-cell chemical lysis in picoliter-scale closed volumes using a microfabricated device. Anal Chem. 2004; 76(20):6137-43. DOI: 10.1021/ac0497508. View

10.

Liu J, Enzelberger M, Quake S . A nanoliter rotary device for polymerase chain reaction. Electrophoresis. 2002; 23(10):1531-6. DOI: 10.1002/1522-2683(200205)23:10<1531::AID-ELPS1531>3.0.CO;2-D. View

11.

Huhmer A, Landers J . Noncontact infrared-mediated thermocycling for effective polymerase chain reaction amplification of DNA in nanoliter volumes. Anal Chem. 2000; 72(21):5507-12. DOI: 10.1021/ac000423j. View

12.

Gascoyne P, Vykoukal J . Particle separation by dielectrophoresis. Electrophoresis. 2002; 23(13):1973-83. PMC: 2726256. DOI: 10.1002/1522-2683(200207)23:13<1973::AID-ELPS1973>3.0.CO;2-1. View

13.

Yeung S, Lee T, Cai H, Hsing I . A DNA biochip for on-the-spot multiplexed pathogen identification. Nucleic Acids Res. 2006; 34(18):e118. PMC: 1636451. DOI: 10.1093/nar/gkl702. View

14.

Easley C, Karlinsey J, Landers J . On-chip pressure injection for integration of infrared-mediated DNA amplification with electrophoretic separation. Lab Chip. 2006; 6(5):601-10. DOI: 10.1039/b600039h. View

15.

Kim J, Jang S, Jia G, Zoval J, Da Silva N, Madou M . Cell lysis on a microfluidic CD (compact disc). Lab Chip. 2004; 4(5):516-22. DOI: 10.1039/b401106f. View

16.

Balaban N, Merrin J, Chait R, Kowalik L, Leibler S . Bacterial persistence as a phenotypic switch. Science. 2004; 305(5690):1622-5. DOI: 10.1126/science.1099390. View

17.

Chabert M, Viovy J . Microfluidic high-throughput encapsulation and hydrodynamic self-sorting of single cells. Proc Natl Acad Sci U S A. 2008; 105(9):3191-6. PMC: 2265149. DOI: 10.1073/pnas.0708321105. View

18.

Cuchacovich R . Clinical applications of the polymerase chain reaction: an update. Infect Dis Clin North Am. 2006; 20(4):735-58, v. DOI: 10.1016/j.idc.2006.09.003. View

19.

Boedicker J, Li L, Kline T, Ismagilov R . Detecting bacteria and determining their susceptibility to antibiotics by stochastic confinement in nanoliter droplets using plug-based microfluidics. Lab Chip. 2008; 8(8):1265-72. PMC: 2612531. DOI: 10.1039/b804911d. View

20.

Mariella Jr R . Sample preparation: the weak link in microfluidics-based biodetection. Biomed Microdevices. 2008; 10(6):777. PMC: 7088034. DOI: 10.1007/s10544-008-9190-7. View