Adaptive Evolution of the Vertebrate Skeletal Muscle Sodium Channel

Overview

Journal Genet Mol Biol

Specialty Genetics

Date 2011 Jul 8

PMID 21734837

Citations 2

Authors

Jian Lu

Jianzhou Zheng

Qinggang Xu

Keping Chen

Chiyu Zhang

Affiliations

Soon will be listed here.

Abstract

Tetrodotoxin (TTX) is a highly potent neurotoxin that blocks the action potential by selectively binding to voltage-gated sodium channels (Na(v)). The skeletal muscle Na(v) (Na(v)1.4) channels in most pufferfish species and certain North American garter snakes are resistant to TTX, whereas in most mammals they are TTX-sensitive. It still remains unclear as to whether the difference in this sensitivity among the various vertebrate species can be associated with adaptive evolution. In this study, we investigated the adaptive evolution of the vertebrate Na(v)1.4 channels. By means of the CODEML program of the PAML 4.3 package, the lineages of both garter snakes and pufferfishes were denoted to be under positive selection. The positively selected sites identified in the p-loop regions indicated their involvement in Na(v)1.4 channel sensitivity to TTX. Most of these sites were located in the intracellular regions of the Na(v)1.4 channel, thereby implying the possible association of these regions with the regulation of voltage-sensor movement.

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References

Yu F, Catterall W . Overview of the voltage-gated sodium channel family. Genome Biol. 2003; 4(3):207. PMC: 153452. DOI: 10.1186/gb-2003-4-3-207. View

Novak A, Jost M, Lu Y, Taylor A, Zakon H, Ribera A . Gene duplications and evolution of vertebrate voltage-gated sodium channels. J Mol Evol. 2006; 63(2):208-21. DOI: 10.1007/s00239-005-0287-9. View

Venkatesh B, Lu S, Dandona N, See S, Brenner S, Soong T . Genetic basis of tetrodotoxin resistance in pufferfishes. Curr Biol. 2005; 15(22):2069-72. DOI: 10.1016/j.cub.2005.10.068. View

Yang Z . PAML 4: phylogenetic analysis by maximum likelihood. Mol Biol Evol. 2007; 24(8):1586-91. DOI: 10.1093/molbev/msm088. View

Narahashi T, Haas H, Therrien E . Saxitoxin and tetrodotoxin: comparison of nerve blocking mechanism. Science. 1967; 157(3795):1441-2. DOI: 10.1126/science.157.3795.1441. View

MOSHER H, FUHRMAN F, BUCHWALD H, FISCHER H . TARICHATOXIN--TETRODOTOXIN: A POTENT NEUROTOXIN. Science. 1964; 144(3622):1100-10. DOI: 10.1126/science.144.3622.1100. View

Tamura K, Dudley J, Nei M, Kumar S . MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007; 24(8):1596-9. DOI: 10.1093/molbev/msm092. View

Felsenstein J . CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP. Evolution. 2017; 39(4):783-791. DOI: 10.1111/j.1558-5646.1985.tb00420.x. View

Marban E, Yamagishi T, Tomaselli G . Structure and function of voltage-gated sodium channels. J Physiol. 1998; 508 ( Pt 3):647-57. PMC: 2230911. DOI: 10.1111/j.1469-7793.1998.647bp.x. View

10.

Lipkind G, Fozzard H . KcsA crystal structure as framework for a molecular model of the Na(+) channel pore. Biochemistry. 2000; 39(28):8161-70. DOI: 10.1021/bi000486w. View

11.

Lopreato G, Lu Y, Southwell A, Atkinson N, Hillis D, Wilcox T . Evolution and divergence of sodium channel genes in vertebrates. Proc Natl Acad Sci U S A. 2001; 98(13):7588-92. PMC: 34712. DOI: 10.1073/pnas.131171798. View

12.

Rohl C, Boeckman F, Baker C, Scheuer T, Catterall W, Klevit R . Solution structure of the sodium channel inactivation gate. Biochemistry. 1999; 38(3):855-61. DOI: 10.1021/bi9823380. View

13.

Geffeney S, Brodie Jr E, Ruben P, Brodie 3rd E . Mechanisms of adaptation in a predator-prey arms race: TTX-resistant sodium channels. Science. 2002; 297(5585):1336-9. DOI: 10.1126/science.1074310. View

14.

Soong T, Venkatesh B . Adaptive evolution of tetrodotoxin resistance in animals. Trends Genet. 2006; 22(11):621-6. DOI: 10.1016/j.tig.2006.08.010. View

15.

Zhang J, Nielsen R, Yang Z . Evaluation of an improved branch-site likelihood method for detecting positive selection at the molecular level. Mol Biol Evol. 2005; 22(12):2472-9. DOI: 10.1093/molbev/msi237. View

16.

Stuhmer W, Conti F, Suzuki H, Wang X, Noda M, Yahagi N . Structural parts involved in activation and inactivation of the sodium channel. Nature. 1989; 339(6226):597-603. DOI: 10.1038/339597a0. View

17.

Goldin A . Evolution of voltage-gated Na(+) channels. J Exp Biol. 2002; 205(Pt 5):575-84. DOI: 10.1242/jeb.205.5.575. View

18.

Geffeney S, Fujimoto E, Brodie 3rd E, Brodie Jr E, Ruben P . Evolutionary diversification of TTX-resistant sodium channels in a predator-prey interaction. Nature. 2005; 434(7034):759-63. DOI: 10.1038/nature03444. View

19.

Thompson J, Higgins D, Gibson T . CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22(22):4673-80. PMC: 308517. DOI: 10.1093/nar/22.22.4673. View