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Deep Tissue Fluorescent Imaging in Scattering Specimens Using Confocal Microscopy

Overview
Journal Microsc Microanal
Publisher Oxford University Press
Date 2011 Jul 7
PMID 21729357
Citations 14
Authors
Sherry G Clendenon
Pamela A Young
Michael Ferkowicz
Carrie Phillips
Kenneth W Dunn
Affiliations
Soon will be listed here.
Abstract

In scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering. We applied the concept of clearing to deep tissue imaging of highly scattering specimens. Clearing is a remarkably effective approach to improving image quality at depth using either confocal or multiphoton microscopy. Tissue clearing appears to eliminate the need for multiphoton excitation for deep tissue imaging.

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