Megapixel Digital PCR

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2011 Jul 5

PMID 21725299

Citations 89

Authors

Kevin A Heyries

Carolina Tropini

Michael VanInsberghe

Callum Doolin

Oleh I Petriv

Anupam Singhal

Kaston Leung

Curtis B Hughesman

Carl L Hansen

Affiliations

Soon will be listed here.

Abstract

We present a microfluidic 'megapixel' digital PCR device that uses surface tension-based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactors cm(-2). This device achieves a dynamic range of 10(7), single-nucleotide-variant detection below one copy per 100,000 wild-type sequences and the discrimination of a 1% difference in chromosome copy number.

Citing Articles

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Overcoming bubble formation in polydimethylsiloxane-made PCR chips: mechanism and elimination with a high-pressure liquid seal.

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SPEED: an integrated, smartphone-operated, handheld digital PCR Device for point-of-care testing.

Zhang H, Liu X, Wang X, Yan Z, Xu Y, Ganova M Microsyst Nanoeng. 2024; 10:62.

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Digital colloid-enhanced Raman spectroscopy by single-molecule counting.

Bi X, Czajkowsky D, Shao Z, Ye J Nature. 2024; 628(8009):771-775.

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