Diagnostic Value of Interferon- Gamma Release Assays (QuantiFERON-TB Gold® In Tube) in Childhood Tuberculosis

Overview

Journal Indian J Pediatr

Specialty Pediatrics

Date 2011 Jun 28

PMID 21706246

Citations 8

Authors

Rajeshwar Dayal

Vikas Verma

B Sharma

G Kumar

N Kumar

R Gupta

V M Katoch

Beenu Joshi

D S Chauhan

Affiliations

Soon will be listed here.

Abstract

Objective: To evaluate the efficacy and diagnostic potential of interferon- gamma release assays QuantiFERON-TB Gold® In Tube (QFT-TB-IT) for the detection of gamma interferon against the antigen ESAT-6, CFP-10, TB 7.7(p4) of Mycobacterium tuberculosis and compare the reactivity with other methods (smear, culture and IS6110 targeting PCR).

Methods: The study was carried out on children with pulmonary and extra pulmonary tuberculosis along with matching and PPD+ controls (82 cases, 48 matching controls and 20 PPD+ controls).

Results: QFT-TB-IT showed a significantly higher sensitivity (51.2%) as compared with LJ medium culture method (11.0%; p < 0.001), BacT/Alert 3D system (12.1%; p < 0.001), ZN staining (19.5%; p < 0.001) and PCR (45.1%; p < 0.05). Specificity of QFT-TB-IT was 48.0%.

Conclusions: Less specificity of this assay may be due to high endemicity of tuberculosis. However, further studies are recommended.

Citing Articles

Comparison of tuberculin skin test and QuantiFERON-TB Gold In-Tube test in Bacillus Calmette-Guerin-vaccinated children.

Shah I, Kathwate J, Shetty N Lung India. 2020; 37(1):24-29.

PMID: 31898617 PMC: 6961094. DOI: 10.4103/lungindia.lungindia_304_19.

Risk Factors for Indeterminate Interferon-Gamma Release Assay for the Diagnosis of Tuberculosis in Children-A Systematic Review and Meta-Analysis.

Meier N, Volken T, Geiger M, Heininger U, Tebruegge M, Ritz N Front Pediatr. 2019; 7:208.

PMID: 31192175 PMC: 6548884. DOI: 10.3389/fped.2019.00208.

Real time PCR in childhood tuberculosis: a valuable diagnostic tool.

Dayal R, Kashyap H, Pounikar G, Kamal R, Yadav N, Singh M Indian J Pediatr. 2014; 82(2):189-91.

PMID: 25029996 DOI: 10.1007/s12098-014-1506-4.

A toolbox for tuberculosis (TB) diagnosis: an Indian multi-centric study (2006-2008); evaluation of serological assays based on PGL-Tb1 and ESAT-6/CFP10 antigens for TB diagnosis.

Lagrange P, Thangaraj S, Dayal R, Deshpande A, Ganguly N, Girardi E PLoS One. 2014; 9(5):e96367.

PMID: 24797271 PMC: 4010510. DOI: 10.1371/journal.pone.0096367.

Systematic review and meta-analysis on the utility of Interferon-gamma release assays for the diagnosis of Mycobacterium tuberculosis infection in children: a 2013 update.

Sollai S, Galli L, De Martino M, Chiappini E BMC Infect Dis. 2014; 14 Suppl 1:S6.

PMID: 24564486 PMC: 4016555. DOI: 10.1186/1471-2334-14-S1-S6.

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