Racial Differences in the Time-Course Oxidative Stress Responses to Acute Exercise

Overview

Journal J Exerc Physiol Online

Date 2011 Jun 22

PMID 21691463

Citations 9

Authors

Deborah L Feairheller

Keith M Diaz

Kathleen M Sturgeon

Sheara T Williamson

Michael D Brown

Affiliations

Soon will be listed here.

Abstract

African Americans have disproportionate levels of cardiovascular disease and oxidative stress. The purpose of our study was to examine racial differences between African American and Caucasian adults in time-course oxidative stress responses to a treadmill test. After a 12-hr fast, 18 participants (9 of each ethnic group; 21 ± 0.4 yrs) completed a submaximal treadmill test and underwent serial blood draws: Pre, Post (within 2 min), 30, 60, and 120 min after exercise. At each time-point, superoxide dismutase (SOD, U/mL), total antioxidant capacity (TAC, mM), protein carbonyls (PC, nmol/mg), and thiobarbituric acid-reactive substance (TBARs, μmol/L) were measured. We found no difference between groups for blood pressure, BMI, or exercise capacity (as measured by volume of oxygen consumed, VO(2) max). African Americans had significantly (p < 0.05) higher SOD (Pre: 5.45 ± 0.4 vs. 3.69 ± 0.69; 60 min: 8.99 ± 0.7 vs. 4.23 ± 0.6; 120 min: 9.69 ± 1.6 vs. 3.52 ± 0.7), TAC (Pre: 2.31 ± 0.25 vs. 1.16 ± 0.3; Post: 2.39 ± 0.2 vs. 1.34 ± 0.2; 30 min: 2.29 ± 0.2 vs. 1.09 ± 0.2), and PC (Pre: 1.09 ± 0.1 vs. 0.82 ± 0.1; Post: 1.14 ± 0.1 vs. 0.81 ± 0.1; 30 min: 1.13 ± 0.1 vs. 0.85 ± 0.1; 60 min: 1.06 ± 0.1 vs. 0.81 ± 0.05), but not TBARs. Between groups, only SOD exhibited a different time-course response: levels for African Americans rose steadily throughout the 120 min, while levels for Caucasians peaked at 30 min and by 120 min had returned to pre-exercise levels. Race had a greater effect on oxidative stress responses than submaximal exercise did. African Americans had significantly higher TAC, SOD, and PC levels compared to Caucasians.

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References

Kerksick C, Taylor 4th L, Harvey A, Willoughby D . Gender-related differences in muscle injury, oxidative stress, and apoptosis. Med Sci Sports Exerc. 2008; 40(10):1772-80. DOI: 10.1249/MSS.0b013e31817d1cce. View

Nikolaidis M, Kyparos A, Hadziioannou M, Panou N, Samaras L, Jamurtas A . Acute exercise markedly increases blood oxidative stress in boys and girls. Appl Physiol Nutr Metab. 2007; 32(2):197-205. DOI: 10.1139/h06-097. View

Christie L, Opii W, Head E, Araujo J, de Rivera C, Milgram N . Short-term supplementation with acetyl-L-carnitine and lipoic acid alters plasma protein carbonyl levels but does not improve cognition in aged beagles. Exp Gerontol. 2009; 44(12):752-9. PMC: 2787885. DOI: 10.1016/j.exger.2009.08.012. View

Demirbag R, Yilmaz R, Guzel S, Celik H, Kocyigit A, Ozcan E . Effects of treadmill exercise test on oxidative/antioxidative parameters and DNA damage. Anadolu Kardiyol Derg. 2006; 6(2):135-40. View

Beal M . Oxidatively modified proteins in aging and disease. Free Radic Biol Med. 2002; 32(9):797-803. DOI: 10.1016/s0891-5849(02)00780-3. View

Buford T, Cooke M, Redd L, Hudson G, Shelmadine B, Willoughby D . Protease supplementation improves muscle function after eccentric exercise. Med Sci Sports Exerc. 2009; 41(10):1908-14. DOI: 10.1249/MSS.0b013e3181a518f0. View

Zitouni K, Nourooz-Zadeh J, Harry D, Kerry S, Betteridge D, Cappuccio F . Race-specific differences in antioxidant enzyme activity in patients with type 2 diabetes: a potential association with the risk of developing nephropathy. Diabetes Care. 2005; 28(7):1698-703. DOI: 10.2337/diacare.28.7.1698. View

Ortenblad N, Madsen K, Djurhuus M . Antioxidant status and lipid peroxidation after short-term maximal exercise in trained and untrained humans. Am J Physiol. 1997; 272(4 Pt 2):R1258-63. DOI: 10.1152/ajpregu.1997.272.4.R1258. View

Covas M, Elosua R, Fito M, Alcantara M, Coca L, Marrugat J . Relationship between physical activity and oxidative stress biomarkers in women. Med Sci Sports Exerc. 2002; 34(5):814-9. DOI: 10.1097/00005768-200205000-00014. View

10.

Mastaloudis A, Leonard S, Traber M . Oxidative stress in athletes during extreme endurance exercise. Free Radic Biol Med. 2001; 31(7):911-22. DOI: 10.1016/s0891-5849(01)00667-0. View

11.

Liu J, Yeo H, Hagen T, Doniger S, Chyu D, Brooks G . Chronically and acutely exercised rats: biomarkers of oxidative stress and endogenous antioxidants. J Appl Physiol (1985). 2000; 89(1):21-8. DOI: 10.1152/jappl.2000.89.1.21. View

12.

Dayan A, Rotstein A, Pinchuk I, Vodovicz A, Lencovski Z, Lichtenberg D . Effect of a short-term graded exhaustive exercise on the susceptibility of serum lipids to oxidation. Int J Sports Med. 2005; 26(9):732-8. DOI: 10.1055/s-2005-837447. View

13.

Winterbourn C, Buss I, Chan T, Plank L, Clark M, Windsor J . Protein carbonyl measurements show evidence of early oxidative stress in critically ill patients. Crit Care Med. 2000; 28(1):143-9. DOI: 10.1097/00003246-200001000-00024. View

14.

Ashmaig M, Starkey B, Ziada A, Amro A, Sobki S, Ferns G . Changes in serum concentration of antioxidants following treadmill exercise testing in patients with suspected ischaemic heart disease. Int J Exp Pathol. 2001; 82(4):243-8. PMC: 2517714. DOI: 10.1111/j.1365-2613.2001.iep0082-0243-x. View

15.

Bloomer R, Davis P, Consitt L, Wideman L . Plasma protein carbonyl response to increasing exercise duration in aerobically trained men and women. Int J Sports Med. 2006; 28(1):21-5. DOI: 10.1055/s-2006-924140. View

16.

Michailidis Y, Jamurtas A, Nikolaidis M, Fatouros I, Koutedakis Y, Papassotiriou I . Sampling time is crucial for measurement of aerobic exercise-induced oxidative stress. Med Sci Sports Exerc. 2007; 39(7):1107-13. DOI: 10.1249/01.mss.0b013e318053e7ba. View

17.

Alessio H, Hagerman A, Fulkerson B, Ambrose J, Rice R, Wiley R . Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc. 2000; 32(9):1576-81. DOI: 10.1097/00005768-200009000-00008. View

18.

Chakravarti B, Chakravarti D . Oxidative modification of proteins: age-related changes. Gerontology. 2006; 53(3):128-39. DOI: 10.1159/000097865. View

19.

Acikgoz O, Aksu I, Topcu A, Kayatekin B . Acute exhaustive exercise does not alter lipid peroxidation levels and antioxidant enzyme activities in rat hippocampus, prefrontal cortex and striatum. Neurosci Lett. 2006; 406(1-2):148-51. DOI: 10.1016/j.neulet.2006.07.034. View

20.

Jenner P . Oxidative stress in Parkinson's disease. Ann Neurol. 2003; 53 Suppl 3:S26-36; discussion S36-8. DOI: 10.1002/ana.10483. View