Vaccination with Human Induced Pluripotent Stem Cells Creates an Antigen-Specific Immune Response Against HIV-1 Gp160

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2011 Jun 21

PMID 21687419

Citations 3

Authors

Shinji Yoshizaki

Mayuko Nishi

Asami Kondo

Yoshitsugu Kojima

Naoki Yamamoto

Akihide Ryo

Affiliations

Soon will be listed here.

Abstract

Induced pluripotent stem cells (iPSCs) are artificially derived from somatic cells that have been transduced with defined reprogramming factors. A previous report has indicated the possibility of using iPSCs as an immune stimulator to generate antigen-specific immunity. In our current study, we have investigated whether human iPSCs (hiPSCs) have the ability to enhance specific immune response against a human immunodeficiency virus type 1 (HIV-1) antigen in a xenogenic mouse model. Our results show that BALB/c mice immunized with hiPSCs transduced with an adenoviral vector encoding HIV-1 gp160 exhibited prominent antigen-specific cellular immune responses. We further found that pre-treatment of hiPSCs with ionizing radiation promotes the secretion of pro-inflammatory cytokines such as interleukin-1 alpha (IL-1α), IL-12, and IL-18. These cytokines might promote the activation of antigen-presenting cells and the effective induction of cellular immunity. Our present findings thus demonstrate that a hiPSCs-based vaccine has the potential to generate cellular immunity against viral antigens such as HIV-1 gp160 in a xenogenic condition.

Citing Articles

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Modelling human disease with pluripotent stem cells.

Siller R, Greenhough S, Park I, Sullivan G Curr Gene Ther. 2013; 13(2):99-110.

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