Reconstituted Basement Membrane (matrigel) and Laminin Can Enhance the Tumorigenicity and the Drug Resistance of Small Cell Lung Cancer Cell Lines

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1990 Sep 1

PMID 2168554

Citations 79

Authors

R Fridman

G Giaccone

T KANEMOTO

G R Martin

A F Gazdar

J L Mulshine

Affiliations

Soon will be listed here.

Abstract

Small cell lung cancer (SCLC) is a fatal malignancy due to its propensity to metastasize widely and to reoccur after chemotherapy in a drug-resistant form. While most SCLC cell lines are anchorage independent for growth, laminin induced the attachment of five of six SCLC cell lines tested (NCI-N417, NCI-H345, NCI-H146, NCI-H187, NCI-H510, and NCI-H209). NCI-N417 SCLC cells adopted a flattened morphology on laminin, and a classic SCLC cell line (NCI-H345) demonstrated a neuron-like appearance while the other SCLC cell lines except NCI-H187 cells, attached but did not spread. Adhesion to laminin was associated with increased resistance to several cytotoxic drugs. Matrigel, an extract of basement membrane proteins, greatly accelerated tumor growth when coinjected with SCLC cells in athymic mice. A synthetic peptide from the B1 chain of laminin, cyclic-YIGSR (Tyr-Ile-Gly-Ser-Arg), inhibited laminin-induced SCLC cell adhesion and migration in vitro and reduced the size of the tumors they formed when coinjected with matrigel and YIGSR. These results suggest that the interaction of SCLC cells with laminin and possibly with other basement membrane proteins can enhance their tumorigenicity and drug resistance.

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References

Kleinman H, McGarvey M, Liotta L, Robey P, Tryggvason K, Martin G . Isolation and characterization of type IV procollagen, laminin, and heparan sulfate proteoglycan from the EHS sarcoma. Biochemistry. 1982; 21(24):6188-93. DOI: 10.1021/bi00267a025. View

Lai S, Goldstein L, Gottesman M, Pastan I, Tsai C, Johnson B . MDR1 gene expression in lung cancer. J Natl Cancer Inst. 1989; 81(15):1144-50. DOI: 10.1093/jnci/81.15.1144. View

McCarthy J, Furcht L . Laminin and fibronectin promote the haptotactic migration of B16 mouse melanoma cells in vitro. J Cell Biol. 1984; 98(4):1474-80. PMC: 2113243. DOI: 10.1083/jcb.98.4.1474. View

Terranova V, Williams J, Liotta L, Martin G . Modulation of the metastatic activity of melanoma cells by laminin and fibronectin. Science. 1984; 226(4677):982-5. DOI: 10.1126/science.6505678. View

Carney D, Gazdar A, Bepler G, Guccion J, Marangos P, Moody T . Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 1985; 45(6):2913-23. View

Carney D, Bunn Jr P, Gazdar A, Pagan J, Minna J . Selective growth in serum-free hormone-supplemented medium of tumor cells obtained by biopsy from patients with small cell carcinoma of the lung. Proc Natl Acad Sci U S A. 1981; 78(5):3185-9. PMC: 319525. DOI: 10.1073/pnas.78.5.3185. View

Gazdar A, Carney D, Sims H, Simmons A . Heterotransplantation of small-cell carcinoma of the lung into nude mice: comparison of intracranial and subcutaneous routes. Int J Cancer. 1981; 28(6):777-83. DOI: 10.1002/ijc.2910280617. View

Terranova V, Liotta L, Russo R, Martin G . Role of laminin in the attachment and metastasis of murine tumor cells. Cancer Res. 1982; 42(6):2265-9. View

Gazdar A, Carney D, Nau M, Minna J . Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res. 1985; 45(6):2924-30. View

10.

McCarthy J, Basara M, Palm S, Sas D, Furcht L . The role of cell adhesion proteins--laminin and fibronectin--in the movement of malignant and metastatic cells. Cancer Metastasis Rev. 1985; 4(2):125-52. DOI: 10.1007/BF00050692. View

11.

Turpeenniemi-Hujanen T, Thorgeirsson U, Rao C, Liotta L . Laminin increases the release of type IV collagenase from malignant cells. J Biol Chem. 1986; 261(4):1883-9. View

12.

Luk G, Baylin S . Anchorage dependency effects on difluoromethylornithine cytotoxicity in human lung carcinoma cells. Cancer Res. 1986; 46(4 Pt 1):1844-8. View

13.

Kleinman H, McGarvey M, Hassell J, Star V, Cannon F, Laurie G . Basement membrane complexes with biological activity. Biochemistry. 1986; 25(2):312-8. DOI: 10.1021/bi00350a005. View

14.

Liotta L, Rao C, Wewer U . Biochemical interactions of tumor cells with the basement membrane. Annu Rev Biochem. 1986; 55:1037-57. DOI: 10.1146/annurev.bi.55.070186.005133. View

15.

Carmichael J, DeGraff W, Gazdar A, Minna J, Mitchell J . Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 1987; 47(4):936-42. View

16.

Graf J, Iwamoto Y, Sasaki M, Martin G, Kleinman H, Robey F . Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding. Cell. 1987; 48(6):989-96. DOI: 10.1016/0092-8674(87)90707-0. View

17.

McLemore T, Liu M, Blacker P, Gregg M, Alley M, ABBOTT B . Novel intrapulmonary model for orthotopic propagation of human lung cancers in athymic nude mice. Cancer Res. 1987; 47(19):5132-40. View

18.

Gunji Y, Gorelik E . Role of fibrin coagulation in protection of murine tumor cells from destruction by cytotoxic cells. Cancer Res. 1988; 48(18):5216-21. View

19.

Kleinman H, Graf J, Iwamoto Y, Sasaki M, Schasteen C, Yamada Y . Identification of a second active site in laminin for promotion of cell adhesion and migration and inhibition of in vivo melanoma lung colonization. Arch Biochem Biophys. 1989; 272(1):39-45. DOI: 10.1016/0003-9861(89)90192-6. View

20.

McCarthy J, Palm S, Furcht L . Migration by haptotaxis of a Schwann cell tumor line to the basement membrane glycoprotein laminin. J Cell Biol. 1983; 97(3):772-7. PMC: 2112555. DOI: 10.1083/jcb.97.3.772. View