Analysis of Endocrine Active and Clinically Silent Corticotropic Adenomas by in Situ Hybridization

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1990 Aug 1

PMID 2167013

Citations 18

Authors

R V Lloyd

K Fields

L Jin

E Horvath

K Kovacs

Affiliations

Soon will be listed here.

Abstract

The distribution of pro-opiomelanocortin (POMC) messenger RNA (mRNA) in 7 functional and 17 clinically silent corticotropic adenomas was analyzed by in situ hybridization (ISH) with 35S-labeled oligonucleotide probes using formalin-fixed paraffin-embedded tissue sections cut from blocks that were in storage between 1 to 14 years. All 7 functional adenomas and 4 subtype 1 tumors had detectable POMC mRNA, while 3 of 6 subtype 2 and 1 of 7 subtype 3 silent adenomas contained detectable POMC mRNA. In situ hybridization analysis with an 35S-labeled beta-actin probe showed a positive hybridization signal in 22 of 22 cases, indicating that the absence of detectable POMC mRNA in some adenomas was not due to loss of the mRNAs during processing of the tissues or because of the age of the embedded tissue blocks. Northern hybridization analysis with the oligonucleotide probes in 2 normal pituitaries and an adenoma causing Cushing's disease detected a 1.2-Kb mRNA in all three tissues, indicating that the oligonucleotide probes were very specific. These results indicate that subtype 1 silent adenomas and clinically active adenomas associated with Cushing's disease contain POMC mRNA that is readily detectable by ISH in routinely processed tissue specimens, while only a few of the subtypes 2 and 3 adenomas have POMC mRNA that can be detected in paraffin blocks with the oligonucleotide probes used in this study.

Citing Articles

Corticotroph (Basophil) invasion of the pars nervosa in the human pituitary: Localization of proopiomelanocortin peptides, galanin and peptidylglycine α-amidating monooxygenase-like immunoreactivities.

Lloyd R, DAmato C, Thiny M, Jin L, Hicks S, Chandler W Endocr Pathol. 2020; 4(2):86-94.

PMID: 32138413 DOI: 10.1007/BF02914457.

Silent corticotroph adenomas.

Cooper O Pituitary. 2014; 18(2):225-31.

PMID: 25534889 PMC: 4376561. DOI: 10.1007/s11102-014-0624-3.

Silent corticogonadotroph adenomas: clinical and cellular characteristics and long-term outcomes.

Cooper O, Ben-Shlomo A, Bonert V, Bannykh S, Mirocha J, Melmed S Horm Cancer. 2010; 1(2):80-92.

PMID: 20717480 PMC: 2921667. DOI: 10.1007/s12672-010-0014-x.

Classification and pathology of pituitary tumors.

Kontogeorgos G Endocrine. 2005; 28(1):27-35.

PMID: 16311407 DOI: 10.1385/ENDO:28:1:027.

Galectin-3 Expression in Functioning and Silent ACTH-Producing Adenomas.

Jin L, Riss D, Ruebel K, Kajita S, Scheithauer B, Horvath E Endocr Pathol. 2005; 16(2):107-14.

PMID: 16199895 DOI: 10.1385/ep:16:2:107.

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