Topoisomerase II Cleavage of Herpes Simplex Virus Type 1 DNA in Vivo is Replication Dependent

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1990 Sep 1

PMID 2166804

Citations 10

Authors

S N Ebert

S S Shtrom

M T Muller

Affiliations

Soon will be listed here.

Abstract

The genome of herpes simplex virus type 1 contains a large number of recognition sites for eucaryotic DNA type II topoisomerase. Topoisomerase II sites were identified by means of the consensus sequence described previously (J.R. Spitzner and M.T. Muller, Nucleic Acids Res. 16:5553-5556, 1988) and then confirmed by sequencing DNA cleavages introduced by purified topoisomerase II. In vivo, host topoisomerase II also introduced double-stranded DNA breaks in the viral genome at sites predicted by the consensus sequence. Host topoisomerase II acted on all immediate-early genes as well as on genes from other temporal classes; however, cleavages were not detected until 4 to 5 h postinfection and were most intense at 10 h postinfection. Topoisomerase II cleavages were not detected when viral DNA replication was prevented with phosphonoacetic acid. These data indicate that, although progeny viral genomes are acted upon by host topoisomerase II, this enzyme either does not act on parental viral genomes before DNA replication or acts on them with such low efficiency that cleavages are beyond our limit of detection. The findings suggest that host topoisomerase II is involved in aspects of viral replication at late times in the infectious cycle.

Citing Articles

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The Epstein-Barr virus deubiquitinating enzyme BPLF1 regulates the activity of topoisomerase II during productive infection.

Li J, Nagy N, Liu J, Gupta S, Frisan T, Hennig T PLoS Pathog. 2021; 17(9):e1009954.

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Tan S, Sim S BMC Med Genomics. 2019; 12(1):9.

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Kaposi's sarcoma-associated herpesvirus ori-Lyt-dependent DNA replication: involvement of host cellular factors.

Wang Y, Li H, Tang Q, Maul G, Yuan Y J Virol. 2008; 82(6):2867-82.

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The neuronal host cell factor-binding protein Zhangfei inhibits herpes simplex virus replication.

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