Desorption Electrospray Ionization Mass Spectrometry for Lipid Characterization and Biological Tissue Imaging

Overview

Journal Biochim Biophys Acta

Specialties Biochemistry
Biophysics

Date 2011 Jun 8

PMID 21645635

Citations 84

Authors

Livia S Eberlin

Christina R Ferreira

Allison L Dill

Demian R Ifa

R Graham Cooks

Affiliations

Soon will be listed here.

Abstract

Desorption electrospray ionization mass spectrometry (DESI-MS) imaging of biological samples allows untargeted analysis and structural characterization of lipids ionized from the near-surface region of a sample under ambient conditions. DESI is a powerful and sensitive MS ionization method for 2D and 3D imaging of lipids from direct and unmodified complex biological samples. This review describes the strengths and limitations of DESI-MS for lipid characterization and imaging together with the technical workflow and a survey of applications. Included are discussions of lipid mapping and biomarker discovery as well as a perspective on the future of DESI imaging.

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