Xylella Fastidiosa Gene Expression Analysis by DNA Microarrays

Overview

Journal Genet Mol Biol

Specialty Genetics

Date 2011 Jun 4

PMID 21637690

Citations 1

Authors

Regiane F Travensolo

Lucia M Carareto-Alves

Maria V C G Costa

Tiago J S Lopes

Emanuel Carrilho

Eliana G M Lemos

Affiliations

Soon will be listed here.

Abstract

Xylella fastidiosa genome sequencing has generated valuable data by identifying genes acting either on metabolic pathways or in associated pathogenicity and virulence. Based on available information on these genes, new strategies for studying their expression patterns, such as microarray technology, were employed. A total of 2,600 primer pairs were synthesized and then used to generate fragments using the PCR technique. The arrays were hybridized against cDNAs labeled during reverse transcription reactions and which were obtained from bacteria grown under two different conditions (liquid XDM(2) and liquid BCYE). All data were statistically analyzed to verify which genes were differentially expressed. In addition to exploring conditions for X. fastidiosa genome-wide transcriptome analysis, the present work observed the differential expression of several classes of genes (energy, protein, amino acid and nucleotide metabolism, transport, degradation of substances, toxins and hypothetical proteins, among others). The understanding of expressed genes in these two different media will be useful in comprehending the metabolic characteristics of X. fastidiosa, and in evaluating how important certain genes are for the functioning and survival of these bacteria in plants.

Citing Articles

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PMID: 24817711 PMC: 4014320. DOI: 10.1107/S2053230X14006578.

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