MicroRNA Expression in the Livers of Inbred Mice

Overview

Journal Mutat Res

Publisher Elsevier

Specialty Genetics

Date 2011 May 28

PMID 21616085

Citations 8

Authors

Daniel M Gatti

Lu Lu

Robert W Williams

Wei Sun

Fred A Wright

David W Threadgill

Ivan Rusyn

Affiliations

Soon will be listed here.

Abstract

MicroRNAs are short, non-coding RNA sequences that regulate genes at the post-transcriptional level and have been shown to be important in development, tissue differentiation, and disease. Limited attention has been given to the natural variation in miRNA expression across genetically diverse populations even though it is well established that genetic polymorphisms can have a profound effect on mRNA levels. Expression level of 577 miRNAs in the livers of 70 strains of inbred mice was assessed, and we found that miRNA expression is highly stable across different strains. Globally, the expression of miRNA target transcripts does not correlate with miRNA expression, primarily due to the low variance of miRNA but high variance of mRNA expression across strains. Our results show that there is little genetic effect on the baseline miRNA levels in murine liver. The stability of mouse liver miRNA expression in a genetically diverse population suggests that treatment-induced disruptions in liver miRNA expression, a phenomenon established for a large number of toxicants, may indicate an important mechanism for the disturbance of normal liver function, and may prove to be a useful genetic background-independent biomarker of toxicant effect.

Citing Articles

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