The Plant G Box Promoter Sequence Activates Transcription in Saccharomyces Cerevisiae and is Bound in Vitro by a Yeast Activity Similar to GBF, the Plant G Box Binding Factor

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1990 Jun 1

PMID 2161333

Citations 32

Authors

R G Donald

U Schindler

A Batschauer

A R Cashmore

Affiliations

Soon will be listed here.

Abstract

G box and I box sequences of the Arabidopsis thaliana ribulose-bisphosphate-1,5-carboxylase small subunit (RBCS) promoter are required for expression mediated by the Arabidopsis rbcS-1A promoter in transgenic tobacco plants and are bound in vitro by factors from plant nuclear extracts termed GBF and GA-1, respectively. We show here that a -390 to -60 rbcS-1A promoter fragment containing the G box and two I boxes activates transcription from a truncated iso-1-cytochrome c (CYC1) gene promoter in Saccharomyces cerevisiae. Mutagenesis of either the rbcS-1A G box or both I box sequences eliminated the expression mediated by this fragment. When polymerized, I box oligonucleotides were also capable of enhancing expression from the truncated CYC1 promoter. Single-copy G box sequences from the Arabidopsis rbcS-1A, Arabidopsis Adh and tomato rbcS-3A promoters were more potent activators and were used in mobility shift assays to identify a DNA binding activity in yeast functionally similar to GBF. In methylation interference experiments, the binding specificity of the yeast protein was indistinguishable from that obtained with plant nuclear extracts.

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References

Ueda Y, Kakimoto S, Oshima Y . Isolation and characterization of acid phosphatase mutants in Saccharomyces cerevisiae. J Bacteriol. 1973; 113(2):727-38. PMC: 285288. DOI: 10.1128/jb.113.2.727-738.1973. View

Gidoni D, Brosio P, Bedbrook J, Dunsmuir P . Novel cis-acting elements in Petunia Cab gene promoters. Mol Gen Genet. 1989; 215(2):337-44. DOI: 10.1007/BF00339739. View

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

Guarente L, Ptashne M . Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1981; 78(4):2199-203. PMC: 319311. DOI: 10.1073/pnas.78.4.2199. View

Ito H, Fukuda Y, Murata K, Kimura A . Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983; 153(1):163-8. PMC: 217353. DOI: 10.1128/jb.153.1.163-168.1983. View

Burke R, Najarian R . The isolation, characterization, and sequence of the pyruvate kinase gene of Saccharomyces cerevisiae. J Biol Chem. 1983; 258(4):2193-201. View

Russell D, Smith M, Williamson V, Young E . Nucleotide sequence of the yeast alcohol dehydrogenase II gene. J Biol Chem. 1983; 258(4):2674-82. View

Arima K, Oshima T, Kubota I, Nakamura N, Mizunaga T, Toh-E A . The nucleotide sequence of the yeast PHO5 gene: a putative precursor of repressible acid phosphatase contains a signal peptide. Nucleic Acids Res. 1983; 11(6):1657-72. PMC: 325827. DOI: 10.1093/nar/11.6.1657. View

Guarente L, Mason T . Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site. Cell. 1983; 32(4):1279-86. DOI: 10.1016/0092-8674(83)90309-4. View

10.

Walter P, Gangloff J, Bonnet J, Boulanger Y, Ebel J, Fasiolo F . Primary structure of the Saccharomyces cerevisiae gene for methionyl-tRNA synthetase. Proc Natl Acad Sci U S A. 1983; 80(9):2437-41. PMC: 393840. DOI: 10.1073/pnas.80.9.2437. View

11.

Bostian K, Lemire J, HALVORSON H . Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae. Mol Cell Biol. 1983; 3(5):839-53. PMC: 368607. DOI: 10.1128/mcb.3.5.839-853.1983. View

12.

Rose M, Botstein D . Construction and use of gene fusions to lacZ (beta-galactosidase) that are expressed in yeast. Methods Enzymol. 1983; 101:167-80. DOI: 10.1016/0076-6879(83)01012-5. View

13.

Guarente L . Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast. Methods Enzymol. 1983; 101:181-91. DOI: 10.1016/0076-6879(83)01013-7. View

14.

Devereux J, Haeberli P, SMITHIES O . A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984; 12(1 Pt 1):387-95. PMC: 321012. DOI: 10.1093/nar/12.1part1.387. View

15.

Meyhack B, Bajwa W, Rudolph H, Hinnen A . Two yeast acid phosphatase structural genes are the result of a tandem duplication and show different degrees of homology in their promoter and coding sequences. EMBO J. 1982; 1(6):675-80. PMC: 553267. DOI: 10.1002/j.1460-2075.1982.tb01229.x. View

16.

Kunkel T . Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A. 1985; 82(2):488-92. PMC: 397064. DOI: 10.1073/pnas.82.2.488. View

17.

Chang C, Meyerowitz E . Molecular cloning and DNA sequence of the Arabidopsis thaliana alcohol dehydrogenase gene. Proc Natl Acad Sci U S A. 1986; 83(5):1408-12. PMC: 323085. DOI: 10.1073/pnas.83.5.1408. View

18.

Chodosh L, Carthew R, Sharp P . A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor. Mol Cell Biol. 1986; 6(12):4723-33. PMC: 367258. DOI: 10.1128/mcb.6.12.4723-4733.1986. View

19.

Wiederrecht G, Shuey D, Kibbe W, Parker C . The Saccharomyces and Drosophila heat shock transcription factors are identical in size and DNA binding properties. Cell. 1987; 48(3):507-15. DOI: 10.1016/0092-8674(87)90201-7. View

20.

Bram R, Kornberg R . Isolation of a Saccharomyces cerevisiae centromere DNA-binding protein, its human homolog, and its possible role as a transcription factor. Mol Cell Biol. 1987; 7(1):403-9. PMC: 365082. DOI: 10.1128/mcb.7.1.403-409.1987. View