Characterization by T1-oligonucleotide Fingerprinting of Three Strains of Human Hepatitis A Virus Isolated in Italy

Overview

Journal Eur J Epidemiol

Specialty Public Health

Date 1990 Mar 1

PMID 2160891

Authors

L Fiore

E De Paolis

F Novello

M Divizia

A Pana

Affiliations

Soon will be listed here.

Abstract

Three human hepatitis A virus strains, all of them isolated in Italy but one acquired abroad, were analyzed by T1-RNAase oligonucleotide mapping and by monoclonal antibody neutralization. The variation among their genomes according to T1-maps was calculated to be about 9%, thus confirming the poor genomic variation assessed by nucleotide sequencing (1-10%). However T1-maps of these Italian isolates were different from those reported in the literature (Weitz and Siegl, 1985). Neutralization by monoclonal antibody caused a reduction in titres of 2-25 log10. This genomic stability, if confirmed, is important with a view to a valuable vaccine.

References

Holland J, Spindler K, Horodyski F, Grabau E, Nichol S, VandePol S . Rapid evolution of RNA genomes. Science. 1982; 215(4540):1577-85. DOI: 10.1126/science.7041255. View

Fiore L, Pierangeli A, Lombardi F, Santoro R, Crainic R, Venuti A . Antigenic and biochemical characterization of poliovirus type 2 isolated from two cases of paralytic disease. Intervirology. 1987; 27(4):196-204. DOI: 10.1159/000149985. View

De Filippis P, Divizia M, Mele A, Adamo B, Pana A . Detection of hepatitis A virus in the stools of healthy people from endemic areas. Eur J Epidemiol. 1987; 3(2):172-5. DOI: 10.1007/BF00239755. View

Linemeyer D, Menke J, Hughes J, Young A, Mitra S . Molecular cloning and partial sequencing of hepatitis A viral cDNA. J Virol. 1985; 54(2):247-55. PMC: 254791. DOI: 10.1128/JVI.54.2.247-255.1985. View

Pedersen F, Haseltine W . Analysis of the genome of an endogenous, ecotropic retrovirus of the AKR strain of mice: micromethod for detailed characterization of high-molecular-weight RNA. J Virol. 1980; 33(1):349-65. PMC: 288552. DOI: 10.1128/JVI.33.1.349-365.1980. View

Domingo E, Sabo D, Taniguchi T, Weissmann C . Nucleotide sequence heterogeneity of an RNA phage population. Cell. 1978; 13(4):735-44. DOI: 10.1016/0092-8674(78)90223-4. View

Lemon S, Binn L . Antigenic relatedness of two strains of hepatitis A virus determined by cross-neutralization. Infect Immun. 1983; 42(1):418-20. PMC: 264574. DOI: 10.1128/iai.42.1.418-420.1983. View

De Wachter R, Fiers W . Preparative two-dimensional polyacrylamide gel electrophoresis of 32 P-labeled RNA. Anal Biochem. 1972; 49(1):184-97. DOI: 10.1016/0003-2697(72)90257-6. View

Baroudy B, Ticehurst J, Miele T, Maizel Jr J, Purcell R, Feinstone S . Sequence analysis of hepatitis A virus cDNA coding for capsid proteins and RNA polymerase. Proc Natl Acad Sci U S A. 1985; 82(7):2143-7. PMC: 397509. DOI: 10.1073/pnas.82.7.2143. View

10.

Weitz M, Siegl G . Variation among hepatitis A virus strains. I. Genomic variation detected by T1 oligonucleotide mapping. Virus Res. 1985; 4(1):53-67. DOI: 10.1016/0168-1702(85)90020-6. View

11.

Najarian R, Caput D, Gee W, Potter S, Renard A, Merryweather J . Primary structure and gene organization of human hepatitis A virus. Proc Natl Acad Sci U S A. 1985; 82(9):2627-31. PMC: 397617. DOI: 10.1073/pnas.82.9.2627. View

12.

Venuti A, Di Russo C, Grosso N, Patti A, Ruggeri F, De Stasio P . Isolation and molecular cloning of a fast-growing strain of human hepatitis A virus from its double-stranded replicative form. J Virol. 1985; 56(2):579-88. PMC: 252615. DOI: 10.1128/JVI.56.2.579-588.1985. View

13.

Cohen J, Ticehurst J, Purcell R, Buckler-White A, Baroudy B . Complete nucleotide sequence of wild-type hepatitis A virus: comparison with different strains of hepatitis A virus and other picornaviruses. J Virol. 1987; 61(1):50-9. PMC: 255199. DOI: 10.1128/JVI.61.1.50-59.1987. View

14.

Pasquini P, KAHN H, Pileggi D, Pana A, Terzi J, DArca T . Prevalence of hepatitis A antibodies in Italy. Int J Epidemiol. 1984; 13(1):83-6. DOI: 10.1093/ije/13.1.83. View

15.

Divizia M, Ruggeri F, Hentschel J, Flehmig B, Pana A . Preliminary characterization of a fast-growing strain of human hepatitis A virus. Microbiologica. 1986; 9(3):269-78. View