Photosynthetic Electron Partitioning Between [FeFe]-hydrogenase and Ferredoxin:NADP+-oxidoreductase (FNR) Enzymes in Vitro

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2011 May 25

PMID 21606330

Citations 43

Authors

Iftach Yacoby

Sergii Pochekailov

Hila Toporik

Maria L Ghirardi

Paul W King

Shuguang Zhang

Affiliations

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Abstract

Photosynthetic water splitting, coupled to hydrogenase-catalyzed hydrogen production, is considered a promising clean, renewable source of energy. It is widely accepted that the oxygen sensitivity of hydrogen production, combined with competition between hydrogenases and NADPH-dependent carbon dioxide fixation are the main limitations for its commercialization. Here we provide evidence that, under the anaerobic conditions that support hydrogen production, there is a significant loss of photosynthetic electrons toward NADPH production in vitro. To elucidate the basis for competition, we bioengineered a ferredoxin-hydrogenase fusion and characterized hydrogen production kinetics in the presence of Fd, ferredoxin:NADP(+)-oxidoreductase (FNR), and NADP(+). Replacing the hydrogenase with a ferredoxin-hydrogenase fusion switched the bias of electron transfer from FNR to hydrogenase and resulted in an increased rate of hydrogen photoproduction. These results suggest a new direction for improvement of biohydrogen production and a means to further resolve the mechanisms that control partitioning of photosynthetic electron transport.

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