The DNA Gyrase of Escherichia Coli Participates in the Formation of a Spontaneous Deletion by RecA-independent Recombination in Vivo

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1990 Feb 1

PMID 2160049

Citations 12

Authors

H Ikeda

Affiliations

Soon will be listed here.

Abstract

A system for detecting a spontaneous deletion in Escherichia coli was developed and the role of DNA gyrase in deletion formation was studied. A derivative of lambda plac5, lambda AM36, was isolated in which whole pBR322 DNA was inserted in the lacZ gene and 227 bp of the lac gene duplicated at both sides of the pBR322 DNA. E. coli lac- strains lysogenized by lambda AM36 had a Lac- phenotype and segregated Lac+ revertants. Sequence analyses showed that the revertant was formed by a deletion that eliminated the inserted pBR322 DNA and one copy of the duplicated segments. The frequency of lac+ revertant formation was independent of recA function, was increased by oxolinic acid, an inhibitor of DNA gyrase, but was not increased in a lysogen of a nalidixic acid-resistant derivative. The reversion frequencies of temperature sensitive mutants of gyrA gene are 10 to 100 times lower than that of the wild-type strain. These results indicate that the DNA gyrase of E. coli participated in the in vivo deletion formation resulting from the direct repeats.

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References

Greaves D, Patient R . RecBC, sbcB independent, (AT)n-mediated deletion of sequences flanking a Xenopus laevis beta globin gene on propagation in E. coli. Nucleic Acids Res. 1986; 14(10):4147-58. PMC: 339851. DOI: 10.1093/nar/14.10.4147. View

Naito A, Naito S, Ikeda H . Homology is not required for recombination mediated by DNA gyrase of Escherichia coli. Mol Gen Genet. 1984; 193(2):238-43. DOI: 10.1007/BF00330674. View

Marvo S, King S, Jaskunas S . Role of short regions of homology in intermolecular illegitimate recombination events. Proc Natl Acad Sci U S A. 1983; 80(9):2452-6. PMC: 393843. DOI: 10.1073/pnas.80.9.2452. View

Farabaugh P, Schmeissner U, Hofer M, Miller J . Genetic studies of the lac repressor. VII. On the molecular nature of spontaneous hotspots in the lacI gene of Escherichia coli. J Mol Biol. 1978; 126(4):847-57. DOI: 10.1016/0022-2836(78)90023-2. View

Gellert M, Mizuuchi K, ODea M, Itoh T, TOMIZAWA J . Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity. Proc Natl Acad Sci U S A. 1977; 74(11):4772-6. PMC: 432037. DOI: 10.1073/pnas.74.11.4772. View

Kourilsky P, Perricaudet M, Gros D, Garapin A, Gottesman M, Fritsch A . Description and properties of bacteriophage lambda vectors useful for the cloning of EcoRI DNA fragments. Biochimie. 1978; 60(2):183-7. DOI: 10.1016/s0300-9084(78)80752-4. View

Kleckner N, Barker D, Ross D, Botstein D . Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda. Genetics. 1978; 90(3):427-61. PMC: 1213899. DOI: 10.1093/genetics/90.3.427. View

Jones I, Primrose S, Ehrlich S . Recombination between short direct repeats in a recA host. Mol Gen Genet. 1982; 188(3):486-9. DOI: 10.1007/BF00330053. View

Kreuzer K, McEntee K, Geballe A, Cozzarelli N . Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations. Mol Gen Genet. 1978; 167(2):129-37. DOI: 10.1007/BF00266906. View

10.

Oka A, Nomura N, Morita M, Sugisaki H, Sugimoto K, TAKANAMI M . Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity. Mol Gen Genet. 1979; 172(2):151-9. DOI: 10.1007/BF00268276. View

11.

Messing J, Vieira J . A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982; 19(3):269-76. DOI: 10.1016/0378-1119(82)90016-6. View

12.

Lopez P, Espinosa M, Greenberg B, Lacks S . Generation of deletions in pneumococcal mal genes cloned in Bacillus subtilis. Proc Natl Acad Sci U S A. 1984; 81(16):5189-93. PMC: 391663. DOI: 10.1073/pnas.81.16.5189. View

13.

Studier F, Rosenberg A, Simon M, Dunn J . Genetic and physical mapping in the early region of bacteriophage T7 DNA. J Mol Biol. 1979; 135(4):917-37. DOI: 10.1016/0022-2836(79)90520-5. View

14.

Steck T, Pruss G, Manes S, Burg L, Drlica K . DNA supercoiling in gyrase mutants. J Bacteriol. 1984; 158(2):397-403. PMC: 215441. DOI: 10.1128/jb.158.2.397-403.1984. View

15.

Messing J . New M13 vectors for cloning. Methods Enzymol. 1983; 101:20-78. DOI: 10.1016/0076-6879(83)01005-8. View

16.

Bergsma D, Olive D, Hartzell S, Byrne B, Subramanian K . Cyclization of linear chimeric plasmids in vivo by a novel end-to-end joining reaction or by intramolecular recombination: one of the products contains a 147-bp perfect palindrome stable in Escherichia coli. Gene. 1982; 20(2):157-67. DOI: 10.1016/0378-1119(82)90034-8. View

17.

Foster T, Lundblad V, Halling S, Kleckner N . Three Tn10-associated excision events: relationship to transposition and role of direct and inverted repeats. Cell. 1981; 23(1):215-27. DOI: 10.1016/0092-8674(81)90286-5. View

18.

Galas D . An analysis of sequence repeats in the lacI gene of Escherichia coli. J Mol Biol. 1978; 126(4):858-63. DOI: 10.1016/0022-2836(78)90024-4. View

19.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

20.

Streisinger G, Owen J . Mechanisms of spontaneous and induced frameshift mutation in bacteriophage T4. Genetics. 1985; 109(4):633-59. PMC: 1202499. DOI: 10.1093/genetics/109.4.633. View