Prospective Evaluation of HSV, Candida Spp., and Oral Bacteria on the Severity of Oral Mucositis in Pediatric Acute Lymphoblastic Leukemia

Overview

Journal Support Care Cancer

Specialties Critical Care
Oncology

Date 2011 May 21

PMID 21597938

Citations 28

Authors

Regina Maria Holanda de Mendonca

Marcela de Araujo

Carlos Emilio Levy

Joseane Morari

Rosangela A Silva

Jose Andres Yunes

Silvia Regina Brandalise

Affiliations

Soon will be listed here.

Abstract

Background: Oral mucositis is a common collateral effect among the secondary complications resulting from chemotherapy. The objective of this study was to prospectively evaluate the association of HSV-1, Candida spp., and oral bacteria on the severity of oral mucositis in pediatric acute lymphoblastic leukemia (ALL).

Procedure: Seventy-one prospective patients were included. Analyses of oral microbiota were conducted on days 14 (D14) and 56 (D56) of the Brazilian GBTLI-99 treatment protocol. Herpes simplex virus (HSV) identification was performed by PCR followed by DNA sequencing analysis. Bacteria and fungi identification was obtained by standard microbiological culture tests.

Results: HSV-1 was found in 10.37% of individual patient samples. One sample was positive for HSV-4. On D14, we found an association between the severity of mucositis and the presence of HSV (p = 0.0347) and Candida spp. (p = 0.0078). At D56, we found an association between the severity of mucositis and the presence of HSV on D14 (p < 0.0001) and HSV presence (p = 0.0317).

Conclusion: The presence of HSV, mainly HSV-1, and Candida spp. was associated with mucositis severity in pediatric ALL. No association could be found between bacterial CFU and severity of mucositis.

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