Mutational Analysis of Cis Elements Involved in E2 Modulation of Human Papillomavirus Type 16 P97 and Type 18 P105 Promoters

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1990 Jun 1

PMID 2159546

Citations 125

Authors

H Romanczuk

F Thierry

P M Howley

Affiliations

Soon will be listed here.

Abstract

cis-Acting elements involved in E2 modulation of human papillomavirus type 16 (HPV-16) P97 promoter activity and HPV-18 P105 promoter activity were examined. In transfected primary human keratinocytes, each promoter had a basal activity that could be repressed by the bovine papillomavirus type 1 E2 gene product. Mutational analysis of the E2-binding sites in the long control region upstream of each promoter revealed that E2 repression was mediated through the E2-binding sites proximal to each promoter. In the context of a mutated E2-binding site at the promoter proximal position, the HPV-16 P97 and HPV-18 P105 promoters could be transactivated by E2. E2-mediated repression of HPV-18 P105 promoter activity was shown to be a transcriptional effect. The interaction of E2 with promoter-proximal E2-binding sites is likely to be important for the controlled expression of viral genes transcribed from the HPV-16 P97 promoter and the HPV-18 P105 promoter in infected human genital epithelial cells.

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References

Durst M, Boukamp P, Fusenig N, Gissmann L . Molecular and cytogenetic analysis of immortalized human primary keratinocytes obtained after transfection with human papillomavirus type 16 DNA. Oncogene. 1987; 1(3):251-6. View

Phelps W, Yee C, Munger K, Howley P . The human papillomavirus type 16 E7 gene encodes transactivation and transformation functions similar to those of adenovirus E1A. Cell. 1988; 53(4):539-47. DOI: 10.1016/0092-8674(88)90570-3. View

Spalholz B, Byrne J, Howley P . Evidence for cooperativity between E2 binding sites in E2 trans-regulation of bovine papillomavirus type 1. J Virol. 1988; 62(9):3143-50. PMC: 253431. DOI: 10.1128/JVI.62.9.3143-3150.1988. View

Storey A, Pim D, Murray A, Osborn K, Banks L, Crawford L . Comparison of the in vitro transforming activities of human papillomavirus types. EMBO J. 1988; 7(6):1815-20. PMC: 457173. DOI: 10.1002/j.1460-2075.1988.tb03013.x. View

Lambert P, Howley P . Bovine papillomavirus type 1 E1 replication-defective mutants are altered in their transcriptional regulation. J Virol. 1988; 62(11):4009-15. PMC: 253829. DOI: 10.1128/JVI.62.11.4009-4015.1988. View

Garcia-Carranca A, Thierry F, Yaniv M . Interplay of viral and cellular proteins along the long control region of human papillomavirus type 18. J Virol. 1988; 62(11):4321-30. PMC: 253867. DOI: 10.1128/JVI.62.11.4321-4330.1988. View

McCance D, Kopan R, Fuchs E, Laimins L . Human papillomavirus type 16 alters human epithelial cell differentiation in vitro. Proc Natl Acad Sci U S A. 1988; 85(19):7169-73. PMC: 282145. DOI: 10.1073/pnas.85.19.7169. View

Schlegel R, Phelps W, Zhang Y, Barbosa M . Quantitative keratinocyte assay detects two biological activities of human papillomavirus DNA and identifies viral types associated with cervical carcinoma. EMBO J. 1988; 7(10):3181-7. PMC: 454709. DOI: 10.1002/j.1460-2075.1988.tb03185.x. View

McBride A, Byrne J, Howley P . E2 polypeptides encoded by bovine papillomavirus type 1 form dimers through the common carboxyl-terminal domain: transactivation is mediated by the conserved amino-terminal domain. Proc Natl Acad Sci U S A. 1989; 86(2):510-4. PMC: 286500. DOI: 10.1073/pnas.86.2.510. View

10.

Gloss B, Chong T, Bernard H . Numerous nuclear proteins bind the long control region of human papillomavirus type 16: a subset of 6 of 23 DNase I-protected segments coincides with the location of the cell-type-specific enhancer. J Virol. 1989; 63(3):1142-52. PMC: 247809. DOI: 10.1128/JVI.63.3.1142-1152.1989. View

11.

Bedell M, Jones K, Grossman S, Laimins L . Identification of human papillomavirus type 18 transforming genes in immortalized and primary cells. J Virol. 1989; 63(3):1247-55. PMC: 247821. DOI: 10.1128/JVI.63.3.1247-1255.1989. View

12.

Dyson N, Howley P, Munger K, Harlow E . The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science. 1989; 243(4893):934-7. DOI: 10.1126/science.2537532. View

13.

Choe J, VAILLANCOURT P, Stenlund A, Botchan M . Bovine papillomavirus type 1 encodes two forms of a transcriptional repressor: structural and functional analysis of new viral cDNAs. J Virol. 1989; 63(4):1743-55. PMC: 248437. DOI: 10.1128/JVI.63.4.1743-1755.1989. View

14.

Schiller J, Kleiner E, Androphy E, Lowy D, Pfister H . Identification of bovine papillomavirus E1 mutants with increased transforming and transcriptional activity. J Virol. 1989; 63(4):1775-82. PMC: 248442. DOI: 10.1128/JVI.63.4.1775-1782.1989. View

15.

Haugen T, Turek L, Mercurio F, Cripe T, Olson B, Anderson R . Sequence-specific and general transcriptional activation by the bovine papillomavirus-1 E2 trans-activator require an N-terminal amphipathic helix-containing E2 domain. EMBO J. 1988; 7(13):4245-53. PMC: 455138. DOI: 10.1002/j.1460-2075.1988.tb03322.x. View

16.

Li R, Knight J, Bream G, Stenlund A, Botchan M . Specific recognition nucleotides and their DNA context determine the affinity of E2 protein for 17 binding sites in the BPV-1 genome. Genes Dev. 1989; 3(4):510-26. DOI: 10.1101/gad.3.4.510. View

17.

Bernard B, Bailly C, Lenoir M, Darmon M, Thierry F, Yaniv M . The human papillomavirus type 18 (HPV18) E2 gene product is a repressor of the HPV18 regulatory region in human keratinocytes. J Virol. 1989; 63(10):4317-24. PMC: 251048. DOI: 10.1128/JVI.63.10.4317-4324.1989. View

18.

Munger K, Phelps W, Bubb V, Howley P, Schlegel R . The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. J Virol. 1989; 63(10):4417-21. PMC: 251060. DOI: 10.1128/JVI.63.10.4417-4421.1989. View

19.

Hawley-Nelson P, Vousden K, Hubbert N, Lowy D, Schiller J . HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes. EMBO J. 1989; 8(12):3905-10. PMC: 402081. DOI: 10.1002/j.1460-2075.1989.tb08570.x. View

20.

Munger K, Werness B, Dyson N, Phelps W, Harlow E, Howley P . Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product. EMBO J. 1989; 8(13):4099-105. PMC: 401588. DOI: 10.1002/j.1460-2075.1989.tb08594.x. View