The Predictive Value of Immunohistochemical Markers in Untreated Wilms' Tumour: Are They Useful?

Overview

Journal World J Urol

Specialty Urology

Date 2011 May 20

PMID 21594709

Citations 4

Authors

Mazen A Ghanem

Theo H van der Kwast

W M Molenaar

Manal A Safan

Rien J Nijman

Gert Jan van Steenbrugge

Affiliations

Soon will be listed here.

Abstract

Purpose: This study reevaluates the potential role of different tumour markers as prognostic indicators in untreated nephroblastoma.

Methods: Expression of a broad panel of tumour markers was investigated by means of immunohistochemical analysis in 43 WT patients. Patients were treated by radical nephrectomy and had a mean follow-up of 11.9 years.

Results: Generally, all the tumour markers studied were expressed in normal kidney tissue and at variable levels in the three cell types of WT (blastema, epithelium and stroma). Immunoreactive blastemal (Bcl-X, Bcl-2 and CD44s) and epithelial (Bcl-X, Bcl-2 and MIB-1) cells were present in the majority of tumours. No correlation was found between their expression and pathological stages. Univariate analysis showed that blastemal WT-1, TGF-α, VEGF, MIB-1 and p27 Kip1 were indicative for clinical progression. In a multivariate analysis, WT-1 protein expression by blastemal cells was an independent prognostic marker for clinical progression.

Conclusions: The blastemal WT-1, TGF-α, VEGF, MIB-1 and p27Kip1 expression correlate with clinical progression in untreated nephroblastoma. Therefore, their expression may be of value in identifying patients with a high propensity to develop distant metastases.

Citing Articles

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Angiogenin expression in human kidneys and Wilms' tumours: relationship with hypoxia and angiogenic factors.

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References

Ghanem M, van der Kwast T, den Hollander J, Sudaryo M, van den Heuvel M, Noordzij M . The prognostic significance of apoptosis-associated proteins BCL-2, BAX and BCL-X in clinical nephroblastoma. Br J Cancer. 2001; 85(10):1557-63. PMC: 2363928. DOI: 10.1054/bjoc.2001.2146. View

Vujanic G, Sandstedt B, Harms D, Kelsey A, Leuschner I, de Kraker J . Revised International Society of Paediatric Oncology (SIOP) working classification of renal tumors of childhood. Med Pediatr Oncol. 2002; 38(2):79-82. DOI: 10.1002/mpo.1276. View

Lemerle J, VOUTE P, Tournade M, Delemarre J, Jereb B, AHSTROM L . Preoperative versus postoperative radiotherapy, single versus multiple courses of actinomycin D, in the treatment of Wilms' tumor. Preliminary results of a controlled clinical trial conducted by the International Society of Paediatric Oncology.... Cancer. 1976; 38(2):647-54. DOI: 10.1002/1097-0142(197608)38:2<647::aid-cncr2820380204>3.0.co;2-c. View

Ghanem M, van der Kwast T, den Hollander J, Sudaryo M, Mathoera R, van den Heuvel M . Expression and prognostic value of epidermal growth factor receptor, transforming growth factor-alpha, and c-erb B-2 in nephroblastoma. Cancer. 2001; 92(12):3120-9. DOI: 10.1002/1097-0142(20011215)92:12<3120::aid-cncr10173>3.0.co;2-2. View

Fridman E, Pinthus J, Kopolovic J, Ramon J, Mor O, Mor Y . Expression of cyclooxygenase-2 in Wilms tumor: immunohistochemical study using tissue microarray methodology. J Urol. 2006; 176(4 Pt 2):1747-50. DOI: 10.1016/j.juro.2006.03.118. View

Ozluk Y, Kilicaslan I, Gulluoglu M, Ayan I, Uysal V . The prognostic significance of angiogenesis and the effect of vascular endothelial growth factor on angiogenic process in Wilms' tumour. Pathology. 2006; 38(5):408-14. DOI: 10.1080/00313020600922926. View

DAngio G, Breslow N, Beckwith J, Evans A, Baum H, DeLorimier A . Treatment of Wilms' tumor. Results of the Third National Wilms' Tumor Study. Cancer. 1989; 64(2):349-60. DOI: 10.1002/1097-0142(19890715)64:2<349::aid-cncr2820640202>3.0.co;2-q. View

Breslow N, Churchill G, Beckwith J, Fernbach D, Otherson H, Tefft M . Prognosis for Wilms' tumor patients with nonmetastatic disease at diagnosis--results of the second National Wilms' Tumor Study. J Clin Oncol. 1985; 3(4):521-31. DOI: 10.1200/JCO.1985.3.4.521. View

de Kraker J, Weitzman S, VOUTE P . Preoperative strategies in the management of Wilms tumor. Hematol Oncol Clin North Am. 1995; 9(6):1275-85. View

10.

Krajewska M, Krajewski S, Epstein J, Shabaik A, Sauvageot J, Song K . Immunohistochemical analysis of bcl-2, bax, bcl-X, and mcl-1 expression in prostate cancers. Am J Pathol. 1996; 148(5):1567-76. PMC: 1861561. View

11.

Hall P, Levison D, Woods A, Yu C, Kellock D, Watkins J . Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol. 1990; 162(4):285-94. DOI: 10.1002/path.1711620403. View

12.

Beckwith J, Palmer N . Histopathology and prognosis of Wilms tumors: results from the First National Wilms' Tumor Study. Cancer. 1978; 41(5):1937-48. DOI: 10.1002/1097-0142(197805)41:5<1937::aid-cncr2820410538>3.0.co;2-u. View

13.

Ghanem M, van Steenbrugge G, Sudaryo M, Mathoera R, Nijman J, Van der Kwast T . Expression and prognostic relevance of vascular endothelial growth factor (VEGF) and its receptor (FLT-1) in nephroblastoma. J Clin Pathol. 2003; 56(2):107-13. PMC: 1769871. DOI: 10.1136/jcp.56.2.107. View

14.

van Leeuwen E, Postma A, Oosterhuis J, Meiring A, Cornelisse C, Koudstaal J . An analysis of histology and DNA-ploidy in primary wilms tumors and their metastases and a study of the morphological effects of therapy. Virchows Arch A Pathol Anat Histopathol. 1987; 410(6):487-94. DOI: 10.1007/BF00781683. View

15.

DAngio G . SIOP (International Society of Paediatric Oncology) and the management of Wilms' tumor. J Clin Oncol. 1983; 1(10):595-6. DOI: 10.1200/JCO.1983.1.10.595. View

16.

Guarda L, Ayala A, Jaffe N, SUTOW W, Bracken R . Chemotherapy-induced histologic changes in Wilms' tumors. Pediatr Pathol. 1984; 2(2):197-206. DOI: 10.3109/15513818409025887. View

17.

Ghanem M, van der Kwast T, Sudaryo M, Mathoera R, van den Heuvel M, Al-Doray A . MIB-1 (KI-67) proliferation index and cyclin-dependent kinase inhibitor p27(Kip1) protein expression in nephroblastoma. Clin Cancer Res. 2004; 10(2):591-7. DOI: 10.1158/1078-0432.ccr-0884-02. View

18.

Ghanem M, van Steenbrugge G, van der Kwast T, Sudaryo M, Noordzij M, Nijman R . Expression and prognostic value Of CD44 isoforms in nephroblastoma (Wilms tumor). J Urol. 2002; 168(2):681-6. View

19.

Kurki P, Vanderlaan M, Dolbeare F, Gray J, Tan E . Expression of proliferating cell nuclear antigen (PCNA)/cyclin during the cell cycle. Exp Cell Res. 1986; 166(1):209-19. DOI: 10.1016/0014-4827(86)90520-3. View

20.

Krajewska M, Fenoglio-Preiser C, Krajewski S, Song K, Macdonald J, Stemmerman G . Immunohistochemical analysis of Bcl-2 family proteins in adenocarcinomas of the stomach. Am J Pathol. 1996; 149(5):1449-57. PMC: 1865280. View