Inflammatory Response in Multiple Organs in a Mouse Model of Acute Alcohol Intoxication and Burn Injury

Overview

Journal J Burn Care Res

Publisher Oxford University Press

Specialty Emergency Medicine

Date 2011 May 20

PMID 21593683

Citations 32

Authors

Xiaoling Li

Suhail Akhtar

Elizabeth J Kovacs

Richard L Gamelli

Mashkoor A Choudhry

Affiliations

Soon will be listed here.

Abstract

This study characterized the inflammatory response after burn injury and determined whether ethanol (EtOH) intoxication at the time of burn injury influences this response. To accomplish this, male mice were gavaged with EtOH (2.9 g/kg) 4 hours before 12 to 15% TBSA sham or burn injury. Mice were killed on day 1 after injury; blood, small intestine, lung, and liver were collected to measure interleukin (IL)-6, IL-10, IL-18, and Monocyte chemotactic protein-1 (MCP-1) levels. In addition, neutrophil infiltration, myeloperoxidase activity, and edema formation were also measured in the small intestine, lung, and liver. There was no difference in the inflammatory markers in the small intestine, lung, and liver in mice receiving either sham or burn injury alone except IL-6 that was increased in all four tissue compartments after burn injury alone. However, when compared with EtOH or burn injury alone, EtOH combined with burn injury resulted in a significant increase in cytokines, neutrophil infiltration, myeloperoxidase activity, and edema in the small intestine, liver, and lung tissue. Furthermore, a significant increase in IL-6 and MCP-1 was observed in circulation after EtOH intoxication and burn injury compared with either EtOH intoxication or burn injury alone; no other cytokines were detected in circulation. These findings suggest that acute EtOH intoxication exacerbates the inflammatory response after burn injury.

Citing Articles

Intoxicated thermal burn injury-mediated systemic immunosuppression involves platelet-activating factor and microvesicle particles.

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Evidence that keratinocyte microvesicle particles carrying platelet-activating factor mediate the widespread multiorgan damage associated with intoxicated thermal burn injury.

Lohade R, Brewer C, Rapp C, Henkels K, Zhang W, Thyagarajan A J Leukoc Biol. 2024; 116(4):766-778.

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Reduced Expression of miR-146a Potentiates Intestinal Inflammation following Alcohol and Burn Injury.

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Transcriptomics, metabolomics, and in-silico drug predictions for liver damage in young and aged burn victims.

Malachowska B, Yang W, Qualman A, Muro I, Boe D, Lampe J Commun Biol. 2023; 6(1):597.

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Differential Effects of Nonsteroidal Anti-Inflammatory Drugs in an In Vitro Model of Human Leaky Gut.

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