A Mechanistic Basis for Potent, Glycoprotein B-directed Gammaherpesvirus Neutralization

Overview

Journal J Gen Virol

Specialty Microbiology

Date 2011 May 20

PMID 21593277

Citations 8

Authors

Daniel L Glauser

Anne-Sophie Kratz

Laurent Gillet

Philip G Stevenson

Affiliations

Soon will be listed here.

Abstract

Glycoprotein B (gB) is a conserved, essential component of gammaherpes virions and so potentially vulnerable to neutralization. However, few good gB-specific neutralizing antibodies have been identified. Here, we show that murid herpesvirus 4 is strongly neutralized by mAbs that recognize an epitope close to one of the gB fusion loops. Antibody binding did not stop gB interacting with its cellular ligands or initiating its fusion-associated conformation change, but did stop gB resolving stably to its post-fusion form, and so blocked membrane fusion to leave virions stranded in late endosomes. The conservation of gB makes this mechanism a possible general route to gammaherpesvirus neutralization.

Citing Articles

The Major Envelope Glycoprotein of Murid Herpesvirus 4 Promotes Sexual Transmission.

Zeippen C, Javaux J, Xiao X, Ledecq M, Mast J, Farnir F J Virol. 2017; 91(13).

PMID: 28424280 PMC: 5469271. DOI: 10.1128/JVI.00235-17.

Deletion of Murid Herpesvirus 4 ORF63 Affects the Trafficking of Incoming Capsids toward the Nucleus.

Latif M, Machiels B, Xiao X, Mast J, Vanderplasschen A, Gillet L J Virol. 2015; 90(5):2455-72.

PMID: 26676769 PMC: 4810702. DOI: 10.1128/JVI.02942-15.

Rhadinovirus host entry by co-operative infection.

Lawler C, Milho R, May J, Stevenson P PLoS Pathog. 2015; 11(3):e1004761.

PMID: 25790477 PMC: 4366105. DOI: 10.1371/journal.ppat.1004761.

Glycoprotein B cleavage is important for murid herpesvirus 4 to infect myeloid cells.

Glauser D, Milho R, Frederico B, May J, Kratz A, Gillet L J Virol. 2013; 87(19):10828-42.

PMID: 23903840 PMC: 3807390. DOI: 10.1128/JVI.00709-13.

Bovine herpesvirus 4 glycoprotein B is indispensable for lytic replication and irreplaceable by VSVg.

Franceschi V, Capocefalo A, Cavirani S, Donofrio G BMC Vet Res. 2013; 9:6.

PMID: 23302472 PMC: 3549454. DOI: 10.1186/1746-6148-9-6.

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