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Species Identification of Aphids (Insecta: Hemiptera: Aphididae) Through DNA Barcodes

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Journal Mol Ecol Resour
Date 2011 May 19
PMID 21586006
Citations 68
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Abstract

A 658-bp fragment of mitochondrial DNA from the 5' region of the mitochondrial cytochrome c oxidase 1 (COI) gene has been adopted as the standard DNA barcode region for animal life. In this study, we test its effectiveness in the discrimination of over 300 species of aphids from more than 130 genera. Most (96%) species were well differentiated, and sequence variation within species was low, averaging just 0.2%. Despite the complex life cycles and parthenogenetic reproduction of aphids, DNA barcodes are an effective tool for identification.

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