A PCR Assay for Gender Assignment in Dugong (Dugong Dugon) and West Indian Manatee (Trichechus Manatus)

Overview

Journal Mol Ecol Resour

Specialties Biology
Environmental Health
Molecular Biology

Date 2011 May 19

PMID 21585866

Citations 6

Authors

M McHale

D Broderick

J R Ovenden

J M Lanyon

Affiliations

Soon will be listed here.

Abstract

Gender assignment for some aquatic mammals in the field is difficult. Molecular sexing from tissue biopsies is possible as males are heterogametic. Here we describe a multiplex PCR assay that amplifies the male specific SRY gene and differentiates ZFX and ZFY gametologues in two sirenian species, dugong (Dugong dugon) and West Indian manatee (Trichechus manatus). The assay was validated with animals of known gender and proved accurate and robust to experimental failure.

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