Guanosine 3',5'-cyclic Nucleotide Binding Proteins of Bovine Retina Identified by Photoaffinity Labeling

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1990 Mar 1

PMID 2156264

Citations 2

Authors

D A Thompson

H G Khorana

Affiliations

Soon will be listed here.

Abstract

Cyclic GMP-binding proteins present in membrane fractions of bovine retina and, in particular, rod outer segments (ROS) were identified by photoaffinity labeling with 8-azido-[32P]cGMP. Two soluble proteins and two membrane-associated proteins were specifically labeled. The soluble proteins, 93 and 72 kDa, corresponded respectively to the alpha subunit of ROS cGMP phosphodiesterase and cGMP-dependent protein kinase. One of the two membrane-associated proteins, 53 kDa, was present in all particulate retinal fractions. Its function is unknown. It is distinct from cAMP-dependent protein kinase or the 63-kDa cGMP-activated channel from ROS. The second membrane-associated protein, 37 kDa, was present only in fractions that did not contain ROS. The molecular mass of this protein was similar to that of a cGMP-binding protein previously attributed to rod cells.

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References

Peterson G . A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem. 1977; 83(2):346-56. DOI: 10.1016/0003-2697(77)90043-4. View

HOLMES R, ROBINS R . PURINE NUCLEOSIDES. IX. THE SYNTHESIS OF 9-BETA-D-RIBOFURANOSYL URIC ACID AND OTHER RELATED 8-SUBSTITUTED PURINE RIBONUCLEOSIDES. J Am Chem Soc. 1965; 87:1772-6. DOI: 10.1021/ja01086a028. View

Farber D, Brown B, Lllley R . Cyclic nucleotide dependent protein kinase and the phosphorylation of endogenous proteins of retinal rod outer segments. Biochemistry. 1979; 18(2):370-8. DOI: 10.1021/bi00569a022. View

Czarnecki J, Geahlen R, Haley B . Synthesis and use of azido photoaffinity analogs of adenine and guanine nucleotides. Methods Enzymol. 1979; 56:642-53. DOI: 10.1016/0076-6879(79)56061-3. View

Potter R, Taylor S . Correlation of the cAMP binding domain with a site of autophosphorylation on the regulatory subunit of cAMP-dependent protein kinase II from porcine skeletal muscle. J Biol Chem. 1979; 254(18):9000-5. View

Baehr W, Devlin M, Applebury M . Isolation and characterization of cGMP phosphodiesterase from bovine rod outer segments. J Biol Chem. 1979; 254(22):11669-77. View

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

Polans A, Hermolin J, Bownds M . Light-induced dephosphorylation of two proteins in frog rod outer segments: influence of cyclic nucleotides and calcium. J Gen Physiol. 1979; 74(5):595-613. PMC: 2228570. DOI: 10.1085/jgp.74.5.595. View

Walter U, Miller P, Wilson F, Menkes D, Greengard P . Immunological distinction between guanosine 3':5'-monophosphate-dependent and adenosine 3':5'-monophosphate-dependent protein kinases. J Biol Chem. 1980; 255(8):3757-62. View

10.

Papermaster D . Preparation of retinal rod outer segments. Methods Enzymol. 1982; 81:48-52. DOI: 10.1016/s0076-6879(82)81010-0. View

11.

Yamazaki A, Bartucca F, Ting A, Bitensky M . Reciprocal effects of an inhibitory factor on catalytic activity and noncatalytic cGMP binding sites of rod phosphodiesterase. Proc Natl Acad Sci U S A. 1982; 79(12):3702-6. PMC: 346494. DOI: 10.1073/pnas.79.12.3702. View

12.

Wilden U, Kuhn H . Light-dependent phosphorylation of rhodopsin: number of phosphorylation sites. Biochemistry. 1982; 21(12):3014-22. DOI: 10.1021/bi00541a032. View

13.

Hurley J, Stryer L . Purification and characterization of the gamma regulatory subunit of the cyclic GMP phosphodiesterase from retinal rod outer segments. J Biol Chem. 1982; 257(18):11094-9. View

14.

Zimmerman A, Yamanaka G, Eckstein F, Baylor D, Stryer L . Interaction of hydrolysis-resistant analogs of cyclic GMP with the phosphodiesterase and light-sensitive channel of retinal rod outer segments. Proc Natl Acad Sci U S A. 1985; 82(24):8813-7. PMC: 391528. DOI: 10.1073/pnas.82.24.8813. View

15.

Stryer L . Cyclic GMP cascade of vision. Annu Rev Neurosci. 1986; 9:87-119. DOI: 10.1146/annurev.ne.09.030186.000511. View

16.

Puckett K, Goldin S . Guanosine 3',5'-cyclic monophosphate stimulates release of actively accumulated calcium in purified disks from rod outer segments of bovine retina. Biochemistry. 1986; 25(7):1739-46. DOI: 10.1021/bi00355a044. View

17.

Cook N, Hanke W, Kaupp U . Identification, purification, and functional reconstitution of the cyclic GMP-dependent channel from rod photoreceptors. Proc Natl Acad Sci U S A. 1987; 84(2):585-9. PMC: 304255. DOI: 10.1073/pnas.84.2.585. View

18.

Nakamura T, Gold G . A cyclic nucleotide-gated conductance in olfactory receptor cilia. Nature. 1987; 325(6103):442-4. DOI: 10.1038/325442a0. View

19.

Matesic D, Liebman P . cGMP-dependent cation channel of retinal rod outer segments. Nature. 1987; 326(6113):600-3. DOI: 10.1038/326600a0. View

20.

Shinozawa T, Sokabe M, Terada S, Matsusaka H, Yoshizawa T . Detection of cyclic GMP binding protein and ion channel activity in frog rod outer segments. J Biochem. 1987; 102(2):281-90. DOI: 10.1093/oxfordjournals.jbchem.a122052. View