The Cytosolic Concentration of Phosphate Determines the Maximal Rate of Glycogenolysis in Perfused Rat Liver

Overview

Journal Biochem J

Specialty Biochemistry

Date 1990 Feb 15

PMID 2155606

Citations 5

Authors

F Vanstapel

M Waebens

P Van Hecke

C Decanniere

W Stalmans

Affiliations

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Abstract

Glycogenolysis was studied in glycogen-rich perfused livers in which glycogen phosphorylase was fully converted into the a form by exposure of the livers to dibutyryl cyclic AMP. We monitored intracellular Pi by 31P n.m.r. Perfusion with Pi-free medium during 30 min caused a progressive decrease of the Pi signal to 50% of its initial value. In contrast, exposure of the livers to KCN and/or 2,4-dinitrophenol resulted in a rapid doubling of the Pi signal. Alterations in the intracellular Pi coincided with proportional changes in the rate of hepatic glycogenolysis (measured as the output of glucose plus lactate). The results indicate that the rate of glycogenolysis catalysed by phosphorylase a depends linearly on the hepatic Pi concentration. Hence the Km of phosphorylase a for its substrate Pi must be considerably higher than the concentrations that occur in the cytosol, even during hypoxia.

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References

Kimura S, Matsumoto T, Tada R, Ogata E, Abe K . Inhibition by calcium channel blockers of the glycogenolytic effect of glucagon in perfused rat liver. Acta Endocrinol (Copenh). 1982; 99(4):559-66. DOI: 10.1530/acta.0.0990559. View

Vandebroeck A, Uyttenhove K, Bollen M, Stalmans W . The hepatic glycogenolysis induced by reversible ischaemia or KCN is exclusively catalysed by phosphorylase a. Biochem J. 1988; 256(2):685-8. PMC: 1135466. DOI: 10.1042/bj2560685. View

Meijer D, Keulemans K, Mulder G . Isolated perfused rat liver technique. Methods Enzymol. 1981; 77:81-94. DOI: 10.1016/s0076-6879(81)77013-7. View

Desmoulin F, Cozzone P, Canioni P . Phosphorus-31 nuclear-magnetic-resonance study of phosphorylated metabolites compartmentation, intracellular pH and phosphorylation state during normoxia, hypoxia and ethanol perfusion, in the perfused rat liver. Eur J Biochem. 1987; 162(1):151-9. DOI: 10.1111/j.1432-1033.1987.tb10555.x. View

HEMS R, Ross B, Berry M, KREBS H . Gluconeogenesis in the perfused rat liver. Biochem J. 1966; 101(2):284-92. PMC: 1270107. DOI: 10.1042/bj1010284. View

Iwai M, Jungermann K . Mechanism of action of cysteinyl leukotrienes on glucose and lactate balance and on flow in perfused rat liver. Comparison with the effects of sympathetic nerve stimulation and noradrenaline. Eur J Biochem. 1989; 180(2):273-81. DOI: 10.1111/j.1432-1033.1989.tb14644.x. View

Dahlqvist A . Determination of maltase and isomaltase activities with a glucose-oxidase reagent. Biochem J. 1961; 80:547-51. PMC: 1243266. DOI: 10.1042/bj0800547. View

Stalmans W, Gevers G . The catalytic activity of phosphorylase b in the liver. With a note on the assay in the glycogenolytic direction. Biochem J. 1981; 200(2):327-36. PMC: 1163539. DOI: 10.1042/bj2000327. View

Murphy E, Gabel S, Funk A, London R . NMR observability of ATP: preferential depletion of cytosolic ATP during ischemia in perfused rat liver. Biochemistry. 1988; 27(2):526-8. DOI: 10.1021/bi00402a003. View

10.

Mclaughlin A, Takeda H, Chance B . Rapid ATP assays in perfused mouse liver by 31P NMR. Proc Natl Acad Sci U S A. 1979; 76(11):5445-9. PMC: 411665. DOI: 10.1073/pnas.76.11.5445. View

11.

Chan T, EXTON J . A rapid method for the determination of glycogen content and radioactivity in small quantities of tissue or isolated hepatocytes. Anal Biochem. 1976; 71(1):96-105. DOI: 10.1016/0003-2697(76)90014-2. View

12.

Vandebroeck A, Bollen M, DE WULF H, Stalmans W . An assessment of the importance of intralysosomal and of alpha-amylolytic glycogenolysis in the liver of normal rats and of rats with a glycogen-storage disease. Eur J Biochem. 1985; 153(3):621-8. DOI: 10.1111/j.1432-1033.1985.tb09345.x. View

13.

Unkefer C, London R . In vivo studies of pyridine nucleotide metabolism in Escherichia coli and Saccharomyces cerevisiae by carbon-13 NMR spectroscopy. J Biol Chem. 1984; 259(4):2311-20. View

14.

Hutson S, Berkich D, Williams G, LaNoue K, Briggs R . 31P NMR visibility and characterization of rat liver mitochondrial matrix adenine nucleotides. Biochemistry. 1989; 28(10):4325-32. DOI: 10.1021/bi00436a030. View

15.

Ogawa S, Rottenberg H, Brown T, Shulman R, Castillo C, Glynn P . High-resolution 31P nuclear magnetic resonance study of rat liver mitochondria. Proc Natl Acad Sci U S A. 1978; 75(4):1796-800. PMC: 392427. DOI: 10.1073/pnas.75.4.1796. View

16.

Van den Berghe G, Hue L, HERS H . Effect of administration of the fructose on the glycogenolytic action of glucagon. An investigation of the pathogeny of hereditary fructose intolerance. Biochem J. 1973; 134(2):637-45. PMC: 1177852. DOI: 10.1042/bj1340637. View

17.

Cohen S, Ogawa S, Rottenberg H, Glynn P, Yamane T, Brown T . P nuclear magnetic resonance studies of isolated rat liver cells. Nature. 1978; 273(5663):554-6. DOI: 10.1038/273554a0. View

18.

HEMS D, Whitton P . Control of hepatic glycogenolysis. Physiol Rev. 1980; 60(1):1-50. DOI: 10.1152/physrev.1980.60.1.1. View

19.

Thoma W, Ugurbil K . pH and compartmentation of isolated perfused rat liver studied by 31P and 19F NMR. NMR Biomed. 1988; 1(2):95-100. DOI: 10.1002/nbm.1940010207. View

20.

Malloy C, Cunningham C, Radda G . The metabolic state of the rat liver in vivo measured by 31P-NMR spectroscopy. Biochim Biophys Acta. 1986; 885(1):1-11. DOI: 10.1016/0167-4889(86)90032-7. View