Modulating F-actin Organization Induces Organ Growth by Affecting the Hippo Pathway

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2011 May 11

PMID 21556047

Citations 249

Authors

Leticia Sansores-Garcia

Wouter Bossuyt

Ken-Ichi Wada

Shigenobu Yonemura

Chunyao Tao

Hiroshi Sasaki

Georg Halder

Affiliations

Soon will be listed here.

Abstract

The Hippo tumour suppressor pathway is a conserved signalling pathway that controls organ size. The core of the Hpo pathway is a kinase cascade, which in Drosophila involves the Hpo and Warts kinases that negatively regulate the activity of the transcriptional coactivator Yorkie. Although several additional components of the Hippo pathway have been discovered, the inputs that regulate Hippo signalling are not fully understood. Here, we report that induction of extra F-actin formation, by loss of Capping proteins A or B, or caused by overexpression of an activated version of the formin Diaphanous, induced strong overgrowth in Drosophila imaginal discs through modulating the activity of the Hippo pathway. Importantly, loss of Capping proteins and Diaphanous overexpression did not significantly affect cell polarity and other signalling pathways, including Hedgehog and Decapentaplegic signalling. The interaction between F-actin and Hpo signalling is evolutionarily conserved, as the activity of the mammalian Yorkie-orthologue Yap is modulated by changes in F-actin. Thus, regulators of F-actin, and in particular Capping proteins, are essential for proper growth control by affecting Hippo signalling.

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