Micronuclei: An Essential Biomarker in Oral Exfoliated Cells for Grading of Oral Squamous Cell Carcinoma

Overview

Journal J Cytol

Specialty Cell Biology

Date 2011 May 10

PMID 21552400

Citations 22

Authors

Kiran Jadhav

Nidhi Gupta

Mujib Br Ahmed

Affiliations

Soon will be listed here.

Abstract

Background: Micronuclei in exfoliated oral epithelial cells have been shown in some studies to correlate with severity of this genotoxic damage. This severity can be measured in terms of grading of the lesions.

Aim: To correlate frequency of micronuclei (MN) in oral exfoliated cells in clinically diagnosed cases of oral squamous cell carcinoma (OSCC) followed by a histopathological grading.

Materials And Methods: The study subjects consisted of clinically diagnosed cases of OSCC. Healthy subjects without any tobacco consumption habits formed the control group. The cytosmears from both groups were stained with rapid Papanicolaou stain. MN were identified according to the criteria given by Countryman and Heddle with some modifications.

Results: The frequency of MN was three to four times higher in patients with OSCC as compared to patients in the control group and the difference was found to be highly significant. In 75% cases, the cytological grade as determined by the frequency of micronuclei correlated with the histopathological grade and this observation was statistically significant.

Conclusions: MN can be a candidate to serve as a biomarker for prediction of the grade of OSCC.

Citing Articles

Genotoxicity and Micronucleus Formation as a Result of Panoramic Radiography in Epithelial Cells of the Buccal Mucosa: A Cross-sectional Study in Adults.

Torabinia N, Mehdizadeh M, Keshani F, Mehdizadeh M, Soltani P, Spagnuolo G Clin Exp Dent Res. 2024; 10(4):e915.

PMID: 39113422 PMC: 11306522. DOI: 10.1002/cre2.915.

Diagnostic Utility of Gram Stain for Oral Smears - A Review.

Vijayakumar T, Divya B, Vasanthi V, Narayan M, Kumar A, Krishnan R J Microsc Ultrastruct. 2023; 11(3):130-134.

PMID: 38025180 PMC: 10679832. DOI: 10.4103/jmau.jmau_108_22.

Nuclear aberrations in the gingival epithelium of patients with chronic periodontitis.

Serikova O, Shumilovich B, Filippova Z, Kalaev V, Kalaeva E, Larina A J Indian Soc Periodontol. 2023; 27(4):374-380.

PMID: 37593557 PMC: 10431217. DOI: 10.4103/jisp.jisp_18_22.

Cytomorphometric Analysis of Oral Buccal Mucosa of Dental Colleges' Students in Sulaimani City.

Mahmood D, Ibraheem B, Mohammad D, Garib B, Hamied M Diagnostics (Basel). 2023; 13(2).

PMID: 36673044 PMC: 9857724. DOI: 10.3390/diagnostics13020234.

The micronucleus assay in the oral exfoliated cells of tannery workers.

Kalim M, Khare A, Shreedhar B, Hadari S, Gupta V, Mahendra A J Oral Maxillofac Pathol. 2020; 23(3):474.

PMID: 31942137 PMC: 6948052. DOI: 10.4103/jomfp.JOMFP_321_18.

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