Increased Expression of Transcription Initiation Factor IIB After Rat Traumatic Brain Injury

Overview

Journal J Mol Histol

Specialty Biochemistry

Date 2011 May 6

PMID 21544596

Citations 12

Authors

Zhiqiang Liu

Donglin Wang

Bai Shao

Xiaohong Wu

Jian Xu

Qiuhui Lu

Yidan Wang

Chunmiao Li

Aiguo Shen

Qiyun Wu

Affiliations

Soon will be listed here.

Abstract

The protein TFIIB is a general transcription initiation factor that plays a pivotal role in the preinitiation complex (PIC) and selects the transcription initiation site. However, its distribution and function in the central nervous system (CNS) remains unclear. In the present study, we mainly investigated the expression and cellular localization of TFIIB during traumatic brain injury (TBI). Western blot analysis revealed that TFIIB was present in normal rat brain cortex. It gradually increased, reached a peak at the 5th day after TBI, and then decreased. Importantly, more TFIIB was colocalized with astrocytes and microglia, which are largely proliferated. In addition, Western blot detection showed that the 5th day post injury was also the proliferation peak indicated by the elevated expression of PCNA. Importantly, injury-induced expression of TFIIB was colabelled by proliferating cell nuclear antigen (proliferating cells marker). These data suggested that TFIIB may be implicated in the proliferation of astrocytes and microglia and the recovery of neurological outcomes. But the inherent mechanisms remained unknown. Further studies are needed to confirm the exact role of TFIIB after brain injury.

Citing Articles

Transcriptional and Translational Regulation of Differentially Expressed Genes in Yucatan Miniswine Brain Tissues following Traumatic Brain Injury.

Rai V, Mendoza-Mari Y, Radwan M, Brazdzionis J, Connett D, Miulli D J Bioinform Syst Biol. 2024; 7(1):81-91.

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Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity.

Haas D, Meiler A, Geiger K, Vogt C, Preuss E, Kochs G PLoS Pathog. 2018; 14(4):e1006980.

PMID: 29709033 PMC: 5927403. DOI: 10.1371/journal.ppat.1006980.

Increased Expression of TBP/TFIID after spinal cord injury in adult rats.

Ding T, Wen H, Wei H, Wu H, Zhao J, Chen F Cell Mol Neurobiol. 2014; 34(5):669-77.

PMID: 24710803 PMC: 11488861. DOI: 10.1007/s10571-014-0048-7.

A new paradigm for transcription factor TFIIB functionality.

Gelev V, Zabolotny J, Lange M, Hiromura M, Yoo S, Orlando J Sci Rep. 2014; 4:3664.

PMID: 24441171 PMC: 3895905. DOI: 10.1038/srep03664.

The expression patterns of Septin-9 after traumatic brain injury in rat brain.

Mao H, Liu J, Shi W, Huang Q, Xu X, Ni L J Mol Neurosci. 2013; 51(2):558-66.

PMID: 23700216 DOI: 10.1007/s12031-013-0024-6.

References

Keane R, Kraydieh S, Lotocki G, Alonso O, Aldana P, Dietrich W . Apoptotic and antiapoptotic mechanisms after traumatic brain injury. J Cereb Blood Flow Metab. 2001; 21(10):1189-98. DOI: 10.1097/00004647-200110000-00007. View

Wieczorek E, Brand M, Jacq X, Tora L . Function of TAF(II)-containing complex without TBP in transcription by RNA polymerase II. Nature. 1998; 393(6681):187-91. DOI: 10.1038/30283. View

LaPlaca M, Simon C, Prado G, Cullen D . CNS injury biomechanics and experimental models. Prog Brain Res. 2007; 161:13-26. DOI: 10.1016/S0079-6123(06)61002-9. View

Somera-Molina K, Robin B, Somera C, Anderson C, Stine C, Koh S . Glial activation links early-life seizures and long-term neurologic dysfunction: evidence using a small molecule inhibitor of proinflammatory cytokine upregulation. Epilepsia. 2007; 48(9):1785-1800. DOI: 10.1111/j.1528-1167.2007.01135.x. View

Ohbayashi T, Makino Y, Tamura T . Identification of a mouse TBP-like protein (TLP) distantly related to the drosophila TBP-related factor. Nucleic Acids Res. 1999; 27(3):750-5. PMC: 148243. DOI: 10.1093/nar/27.3.750. View

Orphanides G, LaGrange T, Reinberg D . The general transcription factors of RNA polymerase II. Genes Dev. 1996; 10(21):2657-83. DOI: 10.1101/gad.10.21.2657. View

Morris G, Mathews M . Regulation of proliferating cell nuclear antigen during the cell cycle. J Biol Chem. 1989; 264(23):13856-64. View

Clark R, Kochanek P, Chen M, Watkins S, Marion D, Chen J . Increases in Bcl-2 and cleavage of caspase-1 and caspase-3 in human brain after head injury. FASEB J. 1999; 13(8):813-21. DOI: 10.1096/fasebj.13.8.813. View

Hochheimer A, Zhou S, Zheng S, Holmes M, Tjian R . TRF2 associates with DREF and directs promoter-selective gene expression in Drosophila. Nature. 2002; 420(6914):439-45. DOI: 10.1038/nature01167. View

10.

Morganti-Kossmann M, Rancan M, Stahel P, Kossmann T . Inflammatory response in acute traumatic brain injury: a double-edged sword. Curr Opin Crit Care. 2002; 8(2):101-5. DOI: 10.1097/00075198-200204000-00002. View

11.

Felzien L, Farrell S, Betts J, Mosavin R, Nabel G . Specificity of cyclin E-Cdk2, TFIIB, and E1A interactions with a common domain of the p300 coactivator. Mol Cell Biol. 1999; 19(6):4241-6. PMC: 104383. DOI: 10.1128/MCB.19.6.4241. View

12.

Shi W, Zhao W, Shen A, Shao B, Wu X, Yang J . Traumatic brain injury induces an up-regulation of Hs1-associated protein X-1 (Hax-1) in rat brain cortex. Neurochem Res. 2010; 36(3):375-82. DOI: 10.1007/s11064-010-0332-y. View

13.

Chen J, Wu X, Shao B, Zhao W, Shi W, Zhang S . Increased expression of TNF receptor-associated factor 6 after rat traumatic brain injury. Cell Mol Neurobiol. 2010; 31(2):269-75. PMC: 11498546. DOI: 10.1007/s10571-010-9617-6. View

14.

Tsuboi A, CONGER K, Garrett K, Conaway R, Conaway J, Arai N . RNA polymerase II initiation factor alpha from rat liver is almost identical to human TFIIB. Nucleic Acids Res. 1992; 20(12):3250. PMC: 312466. DOI: 10.1093/nar/20.12.3250. View

15.

Chirumamilla S, Sun D, Bullock M, Colello R . Traumatic brain injury induced cell proliferation in the adult mammalian central nervous system. J Neurotrauma. 2002; 19(6):693-703. DOI: 10.1089/08977150260139084. View

16.

Buratowski S, Zhou H . Functional domains of transcription factor TFIIB. Proc Natl Acad Sci U S A. 1993; 90(12):5633-7. PMC: 46775. DOI: 10.1073/pnas.90.12.5633. View

17.

Myer V, Young R . RNA polymerase II holoenzymes and subcomplexes. J Biol Chem. 1998; 273(43):27757-60. DOI: 10.1074/jbc.273.43.27757. View

18.

Davidson I . The genetics of TBP and TBP-related factors. Trends Biochem Sci. 2003; 28(7):391-8. DOI: 10.1016/S0968-0004(03)00117-8. View

19.

Moore P, Ozer J, Salunek M, Jan G, Zerby D, Campbell S . A human TATA binding protein-related protein with altered DNA binding specificity inhibits transcription from multiple promoters and activators. Mol Cell Biol. 1999; 19(11):7610-20. PMC: 84787. DOI: 10.1128/MCB.19.11.7610. View

20.

Tatsumi K, Haga S, Matsuyoshi H, Inoue M, Manabe T, Makinodan M . Characterization of cells with proliferative activity after a brain injury. Neurochem Int. 2005; 46(5):381-9. DOI: 10.1016/j.neuint.2004.12.007. View