Early Gene Response of Human Brain Microvascular Endothelial Cells to Listeria Monocytogenes Infection

Overview

Journal Can J Microbiol

Specialty Microbiology

Date 2011 May 6

PMID 21542783

Citations 2

Authors

Chinling Wang

Chung-Hsi Chou

Charles Tseng

Xijin Ge

Lesya M Pinchuk

Affiliations

Soon will be listed here.

Abstract

The gene expression of human brain microvascular endothelial cells (HBMEC) in response to 4 h of infection by Listeria monocytogenes was analyzed. Four hours after infection, the expression of 456 genes of HBMEC had changed (p < 0.05). We noted that many active genes were involved in the formyl-methionyl-leucyl-phenylalanine pathway in infected HBMEC. In the upregulated genes, mRNA levels of interleukin-8 and interleukin-15 in infected cells increased according to microarray and real-time reverse transcription - PCR analyses. Since both cytokines are regarded as potent chemotactic factors, the results suggest that HBMEC are capable of recruiting cells of innate and adaptive immune responses during early L. monocytogenes infection.

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