Sequential Interactions with Sec23 Control the Direction of Vesicle Traffic

Overview

Journal Nature

Specialty Science

Date 2011 May 3

PMID 21532587

Citations 107

Authors

Christopher Lord

Deepali Bhandari

Shekar Menon

Majid Ghassemian

Deborah Nycz

Jesse Hay

Pradipta Ghosh

Susan Ferro-Novick

Affiliations

Soon will be listed here.

Abstract

How the directionality of vesicle traffic is achieved remains an important unanswered question in cell biology. The Sec23p/Sec24p coat complex sorts the fusion machinery (SNAREs) into vesicles as they bud from the endoplasmic reticulum (ER). Vesicle tethering to the Golgi begins when the tethering factor TRAPPI binds to Sec23p. Where the coat is released and how this event relates to membrane fusion is unknown. Here we use a yeast transport assay to demonstrate that an ER-derived vesicle retains its coat until it reaches the Golgi. A Golgi-associated kinase, Hrr25p (CK1δ orthologue), then phosphorylates the Sec23p/Sec24p complex. Coat phosphorylation and dephosphorylation are needed for vesicle fusion and budding, respectively. Additionally, we show that Sec23p interacts in a sequential manner with different binding partners, including TRAPPI and Hrr25p, to ensure the directionality of ER-Golgi traffic and prevent the back-fusion of a COPII vesicle with the ER. These events are conserved in mammalian cells.

Citing Articles

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The Sar1 GTPase is dispensable for COPII-dependent cargo export from the ER.

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