BrainAligner: 3D Registration Atlases of Drosophila Brains

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2011 May 3

PMID 21532582

Citations 76

Authors

Hanchuan Peng

Phuong Chung

Fuhui Long

Lei Qu

Arnim Jenett

Andrew M Seeds

Eugene W Myers

Julie H Simpson

Affiliations

Soon will be listed here.

Abstract

Analyzing Drosophila melanogaster neural expression patterns in thousands of three-dimensional image stacks of individual brains requires registering them into a canonical framework based on a fiducial reference of neuropil morphology. Given a target brain labeled with predefined landmarks, the BrainAligner program automatically finds the corresponding landmarks in a subject brain and maps it to the coordinate system of the target brain via a deformable warp. Using a neuropil marker (the antibody nc82) as a reference of the brain morphology and a target brain that is itself a statistical average of data for 295 brains, we achieved a registration accuracy of 2 μm on average, permitting assessment of stereotypy, potential connectivity and functional mapping of the adult fruit fly brain. We used BrainAligner to generate an image pattern atlas of 2954 registered brains containing 470 different expression patterns that cover all the major compartments of the fly brain.

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