Release of RNA Polymerase from Vero Cell Mitochondria After Herpes Simplex Virus Type 1 Infection

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1990 Jan 1

PMID 2152832

Citations 6

Authors

T Tsurumi

I R Lehman

Affiliations

Soon will be listed here.

Abstract

Infection of Vero cells with herpes simplex virus type 1 results in the appearance in soluble extracts of a DNA primase activity. The partially purified enzyme, Mr, approximately 100,000, is identical in resistance to alpha-amanitin, pH profile, Mg2+ dependence, salt sensitivity, and KmATP to the catalytic core of Vero cell mitochondrial RNA polymerase. Moreover, the products synthesized are those expected of an RNA polymerase rather than a DNA primase. Inasmuch as the enzyme is not present in soluble extracts of uninfected Vero cells, we presume that the specific appearance of RNA polymerase in extracts of herpesvirus-infected cells results from infection-induced disruption of the mitochondrial membrane, followed by release of the enzyme into the cytosol.

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