Structure and Mechanism of the Chromatin Remodelling Factor ISW1a

Overview

Journal Nature

Specialty Science

Date 2011 Apr 29

PMID 21525927

Citations 102

Authors

Kazuhiro Yamada

Timothy D Frouws

Brigitte Angst

Daniel J Fitzgerald

Carl DeLuca

Kyoko Schimmele

David F Sargent

Timothy J Richmond

Affiliations

Soon will be listed here.

Abstract

Site-specific recognition of DNA in eukaryotic organisms depends on the arrangement of nucleosomes in chromatin. In the yeast Saccharomyces cerevisiae, ISW1a and related chromatin remodelling factors are implicated in establishing the nucleosome repeat during replication and altering nucleosome position to affect gene activity. Here we have solved the crystal structures of S. cerevisiae ISW1a lacking its ATPase domain both alone and with DNA bound at resolutions of 3.25 Å and 3.60 Å, respectively, and we have visualized two different nucleosome-containing remodelling complexes using cryo-electron microscopy. The composite X-ray and electron microscopy structures combined with site-directed photocrosslinking analyses of these complexes suggest that ISW1a uses a dinucleosome substrate for chromatin remodelling. Results from a remodelling assay corroborate the dinucleosome model. We show how a chromatin remodelling factor could set the spacing between two adjacent nucleosomes acting as a 'protein ruler'.

Citing Articles

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Angle between DNA linker and nucleosome core particle regulates array compaction revealed by individual-particle cryo-electron tomography.

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General regulatory factors exert differential effects on nucleosome sliding activity of the ISW1a complex.

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ISWI catalyzes nucleosome sliding in condensed nucleosome arrays.

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