Inhibition of Peripheral Blood Neutrophil Oxidative Burst in Periodontitis Patients with a Homeopathic Medication Traumeel S

Overview

Journal Med Sci Monit

Specialties General Medicine
Pathology

Date 2011 Apr 29

PMID 21525811

Citations 4

Authors

Juozas Zilinskas

Jonas Zekonis

Gediminas Zekonis

Renata Sadzeviciene

Marija Sapragoniene

Justina Navickaite

Ingrida Barzdziukaite

Affiliations

Soon will be listed here.

Abstract

Background: The anti-inflammatory effects of a homeopathic remedy, Traumeel S, have been observed in experimental and clinical studies; however, its antioxidant properties have not been elucidated. The aim of the present study was to evaluate the antioxidant effects of Traumeel S on peripheral blood neutrophils in patients with periodontitis.

Material/methods: The study was performed using venous blood of 22 individuals with chronic periodontitis and 21 healthy subjects. The antioxidant effects of Traumeel S on the production of reactive oxygen species by unstimulated and stimulated with unopsonized E. coli neutrophils were investigated using luminol- and lucigenin-dependent chemiluminescence (CL).

Results: Polymorphonuclear leukocytes of periodontitis patients produced higher levels (p<0.01) of light output of lucigenin-dependent chemiluminescence and significantly reduced (p<0.01) light output of luminol-dependent chemiluminescence than analogous cells of healthy subjects. Highly diluted (10⁻⁴ of the stem solution) Traumeel S significantly (by approximately 50%) reduced superoxide-induced oxidation of lucigenin by unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of periodontitis patients and had a tendency to intensify luminol-dependent chemiluminescence. Preincubation of the unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of healthy subjects with Traumeel S exerts no inhibitory action on the luminol- and lucigenin-dependent chemiluminescence of the above-mentioned cells.

Conclusions: This study indicates that Traumeel S may significantly reduce production of superoxide anion by unstimulated and stimulated peripheral blood polymorphonuclear neutrophils of periodontitis patients.

Citing Articles

The Roles of Neutrophils Linking Periodontitis and Atherosclerotic Cardiovascular Diseases.

Irwandi R, Chiesa S, Hajishengallis G, Papayannopoulos V, Deanfield J, DAiuto F Front Immunol. 2022; 13:915081.

PMID: 35874771 PMC: 9300828. DOI: 10.3389/fimmu.2022.915081.

The Natural Combination Medicine Traumeel (Tr14) Improves Resolution of Inflammation by Promoting the Biosynthesis of Specialized Pro-Resolving Mediators.

Jordan P, van Goethem E, Muller A, Hemmer K, Gavioli V, Baillif V Pharmaceuticals (Basel). 2021; 14(11).

PMID: 34832905 PMC: 8623904. DOI: 10.3390/ph14111123.

Testing the nanoparticle-allostatic cross-adaptation-sensitization model for homeopathic remedy effects.

Bell I, Koithan M, Brooks A Homeopathy. 2013; 102(1):66-81.

PMID: 23290882 PMC: 3539158. DOI: 10.1016/j.homp.2012.10.005.

A model for homeopathic remedy effects: low dose nanoparticles, allostatic cross-adaptation, and time-dependent sensitization in a complex adaptive system.

Bell I, Koithan M BMC Complement Altern Med. 2012; 12:191.

PMID: 23088629 PMC: 3570304. DOI: 10.1186/1472-6882-12-191.

References

Oberbaum M, Yaniv I, Ben-Gal Y, Stein J, Ben-Zvi N, Freedman L . A randomized, controlled clinical trial of the homeopathic medication TRAUMEEL S in the treatment of chemotherapy-induced stomatitis in children undergoing stem cell transplantation. Cancer. 2001; 92(3):684-90. DOI: 10.1002/1097-0142(20010801)92:3<684::aid-cncr1371>3.0.co;2-#. View

Pizzo G, Lo Re D, Piscopo M, Pizzo I, Giuliana G . Genetic disorders and periodontal health: a literature review. Med Sci Monit. 2009; 15(8):RA167-78. View

Matthews J, Wright H, Roberts A, Cooper P, Chapple I . Hyperactivity and reactivity of peripheral blood neutrophils in chronic periodontitis. Clin Exp Immunol. 2007; 147(2):255-64. PMC: 1810478. DOI: 10.1111/j.1365-2249.2006.03276.x. View

Schenkein H, Berry C . Activation of complement by Treponema denticola. J Dent Res. 1991; 70(2):107-10. DOI: 10.1177/00220345910700020201. View

Lindena J, Burkhardt H . Separation and chemiluminescence properties of human, canine and rat polymorphonuclear cells. J Immunol Methods. 1988; 115(1):141-7. DOI: 10.1016/0022-1759(88)90321-3. View

Cheson B, CHRISTENSEN R, Sperling R, Kohler B, Babior B . The origin of the chemiluminescence of phagocytosing granulocytes. J Clin Invest. 1976; 58(4):789-96. PMC: 333240. DOI: 10.1172/JCI108530. View

Dahlgren C, Karlsson A . Respiratory burst in human neutrophils. J Immunol Methods. 2000; 232(1-2):3-14. DOI: 10.1016/s0022-1759(99)00146-5. View

Gyllenhammar H . Lucigenin chemiluminescence in the assessment of neutrophil superoxide production. J Immunol Methods. 1987; 97(2):209-13. DOI: 10.1016/0022-1759(87)90461-3. View

Ohkusu K, Isobe K, Hidaka H, Nakashima I . Elucidation of the protein kinase C-dependent apoptosis pathway in distinct subsets of T lymphocytes in MRL-lpr/lpr mice. Eur J Immunol. 1995; 25(11):3180-6. DOI: 10.1002/eji.1830251129. View

10.

Chen H, Cangello D, Benson S, Folmer J, Zhu H, Trush M . Age-related increase in mitochondrial superoxide generation in the testosterone-producing cells of Brown Norway rat testes: relationship to reduced steroidogenic function?. Exp Gerontol. 2001; 36(8):1361-73. DOI: 10.1016/s0531-5565(01)00118-8. View

11.

Bellavite P, Ortolani R, Pontarollo F, Piasere V, Benato G, Conforti A . Immunology and homeopathy. 4. Clinical studies-part 2. Evid Based Complement Alternat Med. 2006; 3(4):397-409. PMC: 1697745. DOI: 10.1093/ecam/nel046. View

12.

Sheppard F, Kelher M, Moore E, McLaughlin N, Banerjee A, Silliman C . Structural organization of the neutrophil NADPH oxidase: phosphorylation and translocation during priming and activation. J Leukoc Biol. 2005; 78(5):1025-42. DOI: 10.1189/jlb.0804442. View

13.

Rosen G, Freeman B . Detection of superoxide generated by endothelial cells. Proc Natl Acad Sci U S A. 1984; 81(23):7269-73. PMC: 392127. DOI: 10.1073/pnas.81.23.7269. View

14.

Porozov S, Cahalon L, Weiser M, Branski D, Lider O, Oberbaum M . Inhibition of IL-1beta and TNF-alpha secretion from resting and activated human immunocytes by the homeopathic medication Traumeel S. Clin Dev Immunol. 2004; 11(2):143-9. PMC: 2270708. DOI: 10.1080/10446670410001722203. View

15.

Andreyev A, Kushnareva Y, Starkov A . Mitochondrial metabolism of reactive oxygen species. Biochemistry (Mosc). 2005; 70(2):200-14. DOI: 10.1007/s10541-005-0102-7. View

16.

Gabrieli C, Kefalas P, Kokkalou E . Antioxidant activity of flavonoids from Sideritis raeseri. J Ethnopharmacol. 2004; 96(3):423-8. DOI: 10.1016/j.jep.2004.09.031. View

17.

Kinane D, Lappin D . Clinical, pathological and immunological aspects of periodontal disease. Acta Odontol Scand. 2001; 59(3):154-60. DOI: 10.1080/000163501750266747. View

18.

Halliwell B, Gutteridge J . The importance of free radicals and catalytic metal ions in human diseases. Mol Aspects Med. 1985; 8(2):89-193. DOI: 10.1016/0098-2997(85)90001-9. View

19.

Oh P, Lim K . Plant originated glycoprotein has anti-oxidative and anti-inflammatory effects on dextran sulfate sodium-induced colitis in mouse. J Biomed Sci. 2006; 13(4):549-60. DOI: 10.1007/s11373-006-9083-9. View

20.

Yang K, Hill H . Neutrophil function disorders: pathophysiology, prevention, and therapy. J Pediatr. 1991; 119(3):343-54. DOI: 10.1016/s0022-3476(05)82044-x. View