Milestones in Candida Albicans Gene Manipulation

Overview

Journal Fungal Genet Biol

Specialties Biology
Genetics
Microbiology

Date 2011 Apr 23

PMID 21511047

Citations 17

Authors

Dhanushki P Samaranayake

Steven D Hanes

Affiliations

Soon will be listed here.

Abstract

In the United States, candidemia is one of the most common hospital-acquired infections and is estimated to cause 10,000 deaths per year. The species Candida albicans is responsible for the majority of these cases. As C. albicans is capable of developing resistance against the currently available drugs, understanding the molecular basis of drug resistance, finding new cellular targets, and further understanding the overall mechanism of C. albicans pathogenesis are important goals. To study this pathogen it is advantageous to manipulate its genome. Numerous strategies of C. albicans gene manipulation have been introduced. This review evaluates a majority of these strategies and should be a helpful guide for researchers to identify gene targeting strategies to suit their requirements.

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References

Liu H, Cottrell T, Pierini L, Goldman W, Doering T . RNA interference in the pathogenic fungus Cryptococcus neoformans. Genetics. 2002; 160(2):463-70. PMC: 1461992. DOI: 10.1093/genetics/160.2.463. View

Gorman J . DNA translocations contribute to chromosome length polymorphisms in Candida albicans. Curr Genet. 1992; 22(2):93-100. DOI: 10.1007/BF00351467. View

De Backer M, Magee P, Pla J . Recent developments in molecular genetics of Candida albicans. Annu Rev Microbiol. 2000; 54:463-98. DOI: 10.1146/annurev.micro.54.1.463. View

Kohler G, White T, Agabian N . Overexpression of a cloned IMP dehydrogenase gene of Candida albicans confers resistance to the specific inhibitor mycophenolic acid. J Bacteriol. 1997; 179(7):2331-8. PMC: 178971. DOI: 10.1128/jb.179.7.2331-2338.1997. View

Fridkin S, Kaufman D, Edwards J, Shetty S, Horan T . Changing incidence of Candida bloodstream infections among NICU patients in the United States: 1995-2004. Pediatrics. 2006; 117(5):1680-7. DOI: 10.1542/peds.2005-1996. View

Wellington M, Kabir M, Rustchenko E . 5-fluoro-orotic acid induces chromosome alterations in genetically manipulated strains of Candida albicans. Mycologia. 2006; 98(3):393-8. DOI: 10.3852/mycologia.98.3.393. View

Noble S, Johnson A . Strains and strategies for large-scale gene deletion studies of the diploid human fungal pathogen Candida albicans. Eukaryot Cell. 2005; 4(2):298-309. PMC: 549318. DOI: 10.1128/EC.4.2.298-309.2005. View

Enloe B, Diamond A, Mitchell A . A single-transformation gene function test in diploid Candida albicans. J Bacteriol. 2000; 182(20):5730-6. PMC: 94694. DOI: 10.1128/JB.182.20.5730-5736.2000. View

Sundstrom P, Cutler J, Staab J . Reevaluation of the role of HWP1 in systemic candidiasis by use of Candida albicans strains with selectable marker URA3 targeted to the ENO1 locus. Infect Immun. 2002; 70(6):3281-3. PMC: 128023. DOI: 10.1128/IAI.70.6.3281-3283.2002. View

10.

Barnett J . A history of research on yeasts 12: medical yeasts part 1, Candida albicans. Yeast. 2008; 25(6):385-417. DOI: 10.1002/yea.1595. View

11.

Santos M, Tuite M . The CUG codon is decoded in vivo as serine and not leucine in Candida albicans. Nucleic Acids Res. 1995; 23(9):1481-6. PMC: 306886. DOI: 10.1093/nar/23.9.1481. View

12.

Magee P, Gale C, Berman J, Davis D . Molecular genetic and genomic approaches to the study of medically important fungi. Infect Immun. 2003; 71(5):2299-309. PMC: 153231. DOI: 10.1128/IAI.71.5.2299-2309.2003. View

13.

Feher T, Karcagi I, Gyorfy Z, Umenhoffer K, Csorgo B, Posfai G . Scarless engineering of the Escherichia coli genome. Methods Mol Biol. 2008; 416:251-9. DOI: 10.1007/978-1-59745-321-9_16. View

14.

Ruhnke M . Epidemiology of Candida albicans infections and role of non-Candida-albicans yeasts. Curr Drug Targets. 2006; 7(4):495-504. DOI: 10.2174/138945006776359421. View

15.

Doyle T, Nawotka K, Purchio A, Akin A, Francis K, Contag P . Expression of firefly luciferase in Candida albicans and its use in the selection of stable transformants. Microb Pathog. 2006; 40(2):69-81. DOI: 10.1016/j.micpath.2005.11.002. View

16.

Miceli M, Diaz J, Lee S . Emerging opportunistic yeast infections. Lancet Infect Dis. 2011; 11(2):142-51. DOI: 10.1016/S1473-3099(10)70218-8. View

17.

Epstein J, Hancock P, Nantel S . Oral candidiasis in hematopoietic cell transplantation patients: an outcome-based analysis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 96(2):154-63. DOI: 10.1016/s1079-2104(03)00296-8. View

18.

Park Y, Morschhauser J . Tetracycline-inducible gene expression and gene deletion in Candida albicans. Eukaryot Cell. 2005; 4(8):1328-42. PMC: 1214539. DOI: 10.1128/EC.4.8.1328-1342.2005. View

19.

Muller F, Groll A, Walsh T . Current approaches to diagnosis and treatment of fungal infections in children infected with human immuno deficiency virus. Eur J Pediatr. 1999; 158(3):187-99. DOI: 10.1007/s004310051047. View

20.

Davis D, Edwards Jr J, Mitchell A, Ibrahim A . Candida albicans RIM101 pH response pathway is required for host-pathogen interactions. Infect Immun. 2000; 68(10):5953-9. PMC: 101559. DOI: 10.1128/IAI.68.10.5953-5959.2000. View