Neurogenesis of Adipose-derived Stem Cells in Hydrogel

Overview

Journal J Huazhong Univ Sci Technolog Med Sci

Specialty General Medicine

Date 2011 Apr 21

PMID 21505979

Citations 4

Authors

Xuewei Xie

Zhouping Tang

Juan Chen

Jie Yang

Wengao Zeng

Na Liu

Yongming Liu

Affiliations

Soon will be listed here.

Abstract

Adipose tissue is a readily available source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Peptide hydrogel is a novel biomaterial which provides three-dimensional microenvironments for a variety of cells for tissue grafting. In this study, adipose-derived stem cells (ADSCs) were isolated from rats, seeded into the peptide hydrogel polymer scaffolds and cultured in Neurobasal (NB) media supplemented with B27, basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Ten days after the culture, some cells were expanded into clonal populations in which the expression of both Nestin and Brdu was detected but only Brdu expression was detected in the cells that were not expanded into clonal populations. Our results suggested that ADSCs in peptide hydrogel polymer scaffolds can be induced to differentiate into cells capable of expressing the neuron-associated markers, self-renewal and self-propagation.

Citing Articles

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Changes of neural markers expression during late neurogenic differentiation of human adipose-derived stem cells.

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Time-Dependent Effect of Encapsulating Alginate Hydrogel on Neurogenic Potential.

Razavi S, Khosravizadeh Z, Bahramian H, Kazemi M Cell J. 2015; 17(2):304-11.

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An improved cryosection method for polyethylene glycol hydrogels used in tissue engineering.

Ruan J, Tulloch N, Muskheli V, Genova E, Mariner P, Anseth K Tissue Eng Part C Methods. 2013; 19(10):794-801.

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