Structure-guided Redesign of D-fructose-6-phosphate Aldolase from E. Coli: Remarkable Activity and Selectivity Towards Acceptor Substrates by Two-point Mutation

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2011 Apr 19

PMID 21499643

Citations 10

Authors

Mariana Gutierrez

Teodor Parella

Jesus Joglar

Jordi Bujons

Pere Clapes

Affiliations

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Abstract

Structure-guided re-design of the acceptor binding site of D-fructose-6-phosphate aldolase from E. coli leads to the construction of FSA A129S/A165G double mutant with an activity between 5- to >900-fold higher than that of wild-type towards N-Cbz-aminoaldehyde derivatives.

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