Endocytic Membrane Fusion and Buckling-induced Microtubule Severing Mediate Cell Abscission

Overview

Journal J Cell Sci

Specialty Cell Biology

Date 2011 Apr 14

PMID 21486954

Citations 57

Authors

John A Schiel

Kristin Park

Mary K Morphew

Evan Reid

Andreas Hoenger

Rytis Prekeris

Affiliations

Soon will be listed here.

Abstract

Cytokinesis and abscission are complicated events that involve changes in membrane transport and cytoskeleton organization. We have used the combination of time-lapse microscopy and correlative high-resolution 3D tomography to analyze the regulation and spatio-temporal remodeling of endosomes and microtubules during abscission. We show that abscission is driven by the formation of a secondary ingression within the intracellular bridge connecting two daughter cells. The initiation and expansion of this secondary ingression requires recycling endosome fusion with the furrow plasma membrane and nested central spindle microtubule severing. These changes in endosome fusion and microtubule reorganization result in increased intracellular bridge plasma membrane dynamics and abscission. Finally, we show that central spindle microtubule reorganization is driven by localized microtubule buckling and breaking, rather than by spastin-dependent severing. Our results provide a new mechanism for mediation and regulation of the abscission step of cytokinesis.

Citing Articles

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Cell intrinsic mechanical regulation of plasma membrane accumulation at the cytokinetic furrow.

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