Gene Expression Profiles in Rat Mesenteric Lymph Nodes Upon Supplementation with Conjugated Linoleic Acid During Gestation and Suckling

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2011 Apr 13

PMID 21481241

Citations 3

Authors

Elisabet Selga

Francisco J Perez-Cano

Angels Franch

Carolina Ramirez-Santana

Montserrat Rivero

Carlos J Ciudad

Cristina Castellote

Veronique Noe

Affiliations

Soon will be listed here.

Abstract

Background: Diet plays a role on the development of the immune system, and polyunsaturated fatty acids can modulate the expression of a variety of genes. Human milk contains conjugated linoleic acid (CLA), a fatty acid that seems to contribute to immune development. Indeed, recent studies carried out in our group in suckling animals have shown that the immune function is enhanced after feeding them with an 80:20 isomer mix composed of c9,t11 and t10,c12 CLA. However, little work has been done on the effects of CLA on gene expression, and even less regarding immune system development in early life.

Results: The expression profile of mesenteric lymph nodes from animals supplemented with CLA during gestation and suckling through dam's milk (Group A) or by oral gavage (Group B), supplemented just during suckling (Group C) and control animals (Group D) was determined with the aid of the specific GeneChip(®) Rat Genome 230 2.0 (Affymettrix). Bioinformatics analyses were performed using the GeneSpring GX software package v10.0.2 and lead to the identification of 89 genes differentially expressed in all three dietary approaches. Generation of a biological association network evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network. Gene underexpression was confirmed by Real-Time RT-PCR.

Conclusions: Ctgf, Timp1, Gal and Syt1, among others, are genes modulated by CLA supplementation that may have a role on mucosal immune responses in early life.

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References

Ramirez-Santana C, Castellote C, Castell M, Molto-Puigmarti C, Rivero M, Perez-Cano F . Enhancement of antibody synthesis in rats by feeding cis-9,trans-11 conjugated linoleic acid during early life. J Nutr Biochem. 2010; 22(5):495-501. DOI: 10.1016/j.jnutbio.2010.04.005. View

Ip M, Shoemaker S, Ip C . Conjugated linoleic acid inhibits proliferation and induces apoptosis of normal rat mammary epithelial cells in primary culture. Exp Cell Res. 1999; 250(1):22-34. DOI: 10.1006/excr.1999.4499. View

Brose N, Petrenko A, Sudhof T, Jahn R . Synaptotagmin: a calcium sensor on the synaptic vesicle surface. Science. 1992; 256(5059):1021-5. DOI: 10.1126/science.1589771. View

Textor B, Licht A, Tuckermann J, Jessberger R, Razin E, Angel P . JunB is required for IgE-mediated degranulation and cytokine release of mast cells. J Immunol. 2007; 179(10):6873-80. DOI: 10.4049/jimmunol.179.10.6873. View

Field C, Clandinin M, Van Aerde J . Polyunsaturated fatty acids and T-cell function: implications for the neonate. Lipids. 2001; 36(9):1025-32. DOI: 10.1007/s11745-001-0813-6. View

Del Rio M, Hernanz A, de la Fuente M . Bombesin, gastrin-releasing peptide, and neuromedin C modulate murine lymphocyte proliferation through adherent accessory cells and activate protein kinase C. Peptides. 1994; 15(1):15-22. DOI: 10.1016/0196-9781(94)90164-3. View

Reeves P, Nielsen F, Fahey Jr G . AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993; 123(11):1939-51. DOI: 10.1093/jn/123.11.1939. View

LANG J, Fukuda M, Zhang H, Mikoshiba K, Wollheim C . The first C2 domain of synaptotagmin is required for exocytosis of insulin from pancreatic beta-cells: action of synaptotagmin at low micromolar calcium. EMBO J. 1997; 16(19):5837-46. PMC: 1170215. DOI: 10.1093/emboj/16.19.5837. View

Geppert M, Goda Y, Hammer R, Li C, Rosahl T, Stevens C . Synaptotagmin I: a major Ca2+ sensor for transmitter release at a central synapse. Cell. 1994; 79(4):717-27. DOI: 10.1016/0092-8674(94)90556-8. View

10.

Pariza M, Park Y, Cook M . Mechanisms of action of conjugated linoleic acid: evidence and speculation. Proc Soc Exp Biol Med. 2000; 223(1):8-13. DOI: 10.1046/j.1525-1373.2000.22302.x. View

11.

Mouta Carreira C, Lavallee T, Tarantini F, Jackson A, Lathrop J, Hampton B . S100A13 is involved in the regulation of fibroblast growth factor-1 and p40 synaptotagmin-1 release in vitro. J Biol Chem. 1998; 273(35):22224-31. DOI: 10.1074/jbc.273.35.22224. View

12.

Hubner N, Wallace C, Zimdahl H, Petretto E, Schulz H, Maciver F . Integrated transcriptional profiling and linkage analysis for identification of genes underlying disease. Nat Genet. 2005; 37(3):243-53. DOI: 10.1038/ng1522. View

13.

Nuttall R, Sampieri C, Pennington C, Gill S, Schultz G, Edwards D . Expression analysis of the entire MMP and TIMP gene families during mouse tissue development. FEBS Lett. 2004; 563(1-3):129-34. DOI: 10.1016/S0014-5793(04)00281-9. View

14.

Koh T, Bellen H . Synaptotagmin I, a Ca2+ sensor for neurotransmitter release. Trends Neurosci. 2003; 26(8):413-22. DOI: 10.1016/S0166-2236(03)00195-4. View

15.

Rachfal A, Brigstock D . Structural and functional properties of CCN proteins. Vitam Horm. 2005; 70:69-103. DOI: 10.1016/S0083-6729(05)70003-0. View

16.

Neusser M, Kraus A, Regele H, Cohen C, Fehr T, Kerjaschki D . The chemokine receptor CXCR7 is expressed on lymphatic endothelial cells during renal allograft rejection. Kidney Int. 2010; 77(9):801-8. DOI: 10.1038/ki.2010.6. View

17.

Sanderson I, Naik S . Dietary regulation of intestinal gene expression. Annu Rev Nutr. 2000; 20:311-38. DOI: 10.1146/annurev.nutr.20.1.311. View

18.

Mutch D, Grigorov M, Berger A, Fay L, Roberts M, Watkins S . An integrative metabolism approach identifies stearoyl-CoA desaturase as a target for an arachidonate-enriched diet. FASEB J. 2005; 19(6):599-601. DOI: 10.1096/fj.04-2674fje. View

19.

Hayashi A, de Medeiros S, Carvalho M, Lanna D . Conjugated linoleic acid (CLA) effects on pups growth, milk composition and lipogenic enzymes in lactating rats. J Dairy Res. 2007; 74(2):160-6. DOI: 10.1017/S002202990600224X. View

20.

Sugimoto T, Seki N, Shimizu S, Kikkawa N, Tsukada J, Shimada H . The galanin signaling cascade is a candidate pathway regulating oncogenesis in human squamous cell carcinoma. Genes Chromosomes Cancer. 2008; 48(2):132-42. DOI: 10.1002/gcc.20626. View