High-throughput Approaches for Plant Epigenomic Studies

Overview

Journal Curr Opin Plant Biol

Specialty Biology

Date 2011 Apr 8

PMID 21470901

Citations 23

Authors

Robert J Schmitz

Xiaoyu Zhang

Affiliations

Soon will be listed here.

Abstract

In plant cells, DNA is packaged into chromatin by wrapping around histone octamers. Pathways that lead to cytosine DNA methylation, posttranslational histone modifications and certain components of the RNA interfering (RNAi) pathway are critically important in modulating chromatin structure, thereby affecting many molecular processes that take place in a cell. Recent advances in microarray and high-throughput sequencing technologies have made it possible to study these pathways on a genome-wide scale. Results from such epigenomic studies are broadening our understanding of plant genomes and are also providing important clues regarding the mechanisms and functions of these pathways that can be further tested using genetic and biochemical approaches. This review focuses on the high-throughput approaches that have been successfully applied in plant epigenomic studies.

Citing Articles

The pattern of alternative splicing and DNA methylation alteration and their interaction in linseed (Linum usitatissimum L.) response to repeated drought stresses.

Wang L, Wang L, Tan M, Wang L, Zhao W, You J Biol Res. 2023; 56(1):12.

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Cataloging Posttranslational Modifications in Plant Histones.

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Identification of epigenetic variation associated with synchronous pod maturity in mungbean (Vigna radiata L.).

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DNA methylation footprints during soybean domestication and improvement.

Shen Y, Zhang J, Liu Y, Liu S, Liu Z, Duan Z Genome Biol. 2018; 19(1):128.

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Methylation Sensitive Amplification Polymorphism Sequencing (MSAP-Seq)-A Method for High-Throughput Analysis of Differentially Methylated CCGG Sites in Plants with Large Genomes.

Chwialkowska K, Korotko U, Kosinska J, Szarejko I, Kwasniewski M Front Plant Sci. 2017; 8:2056.

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