Cell Response to Collagen-calcium Phosphate Cement Scaffolds Investigated for Nonviral Gene Delivery

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2011 Apr 5

PMID 21461916

Citations 5

Authors

R A Perez

M P Ginebra

M Spector

Affiliations

Soon will be listed here.

Abstract

Collagen-hydroxyapatite (HA) scaffolds for the non-viral delivery of a plasmid encoding the osteoinductive protein bone morphogenetic protein (BMP)-7 were developed. The collagen-HA was obtained by the combination of calcium phosphate cement in a collagen template. The effect on cell behavior of increasing amounts of HA in the scaffolds was evaluated. Collagen-HA scaffolds containing 13, 23 or 83 wt% HA were prepared. Cell proliferation was reduced in the 83% HA scaffold after 1 day compared to 13 and 23% HA, but by 14 days the number of cells in 83% HA considerably increased. Alkaline phosphatase (ALP) activity was 8 times higher for the 83% HA scaffolds. BMP-7 plasmid was incorporated into the 83% HA scaffold. The transfection was low, although significant levels of BMP7 were expressed, associated with an increase in cell proliferation.

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Injectable collagen/α-tricalcium phosphate cement: collagen-mineral phase interactions and cell response.

Perez R, Ginebra M J Mater Sci Mater Med. 2012; 24(2):381-93.

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Polymeric additives to enhance the functional properties of calcium phosphate cements.

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