Antipathogenic Potential of Marine Bacillus Sp. SS4 on N-acyl-homoserine-lactone-mediated Virulence Factors Production in Pseudomonas Aeruginosa (PAO1)

Overview

Journal J Biosci

Specialties Biochemistry
Biology

Date 2011 Apr 1

PMID 21451248

Citations 28

Authors

K Syed Musthafa

V Saroja

S Karutha Pandian

A Veera Ravi

Affiliations

Soon will be listed here.

Abstract

Antipathogenic therapy is an outcome of the quorum-sensing inhibition (QSI) mechanism, which targets autoinducer-dependent virulent gene expression in bacterial pathogens. N-acyl homoserine lactone (AHL) acts as a key regulator in the production of virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1 and violacein pigment production in Chromobacterium violaceum. In the present study, the marine bacterial strain SS4 showed potential QSI activity in a concentration-dependent manner (0.5-2 mg/ml) against the AHL-mediated violacein production in C. violaceum (33-86%) and biofilm formation (33-88%), total protease (20-65%), LasA protease (59-68%), LasB elastase (36-68%), pyocyanin (17-86%) and pyoverdin productions in PAO1. The light and confocal laser scanning microscopic analyses confirmed the reduction of the biofilm-forming ability of PAO1 when treated with SS4 extract. Furthermore, the antibiofilm potential was confirmed through static biofilm ring assay, in which ethyl acetate extract of SS4 showed concentration-dependent reduction in the biofilm-forming ability of PAO1. Thus, the result of this study clearly reveals the antipathogenic and antibiofilm properties of the bacterial isolate SS4. Through 16S rDNA analysis, the strain SS4 was identified as Bacillus sp. (GenBank Accession Number: GU471751).

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References

Thenmozhi R, Nithyanand P, Rathna J, Pandian S . Antibiofilm activity of coral-associated bacteria against different clinical M serotypes of Streptococcus pyogenes. FEMS Immunol Med Microbiol. 2009; 57(3):284-94. DOI: 10.1111/j.1574-695X.2009.00613.x. View

Essar D, Eberly L, Hadero A, Crawford I . Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications. J Bacteriol. 1990; 172(2):884-900. PMC: 208517. DOI: 10.1128/jb.172.2.884-900.1990. View

Pesci E, Pearson J, Seed P, Iglewski B . Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa. J Bacteriol. 1997; 179(10):3127-32. PMC: 179088. DOI: 10.1128/jb.179.10.3127-3132.1997. View

Hentzer M, Wu H, Andersen J, Riedel K, Rasmussen T, Bagge N . Attenuation of Pseudomonas aeruginosa virulence by quorum sensing inhibitors. EMBO J. 2003; 22(15):3803-15. PMC: 169039. DOI: 10.1093/emboj/cdg366. View

Bjarnsholt T, Jensen P, Rasmussen T, Christophersen L, Calum H, Hentzer M . Garlic blocks quorum sensing and promotes rapid clearing of pulmonary Pseudomonas aeruginosa infections. Microbiology (Reading). 2005; 151(Pt 12):3873-3880. DOI: 10.1099/mic.0.27955-0. View

Lieberman D, Lieberman D . Pseudomonal infections in patients with COPD: epidemiology and management. Am J Respir Med. 2004; 2(6):459-68. DOI: 10.1007/BF03256673. View

Bjarnsholt T, Givskov M . Quorum-sensing blockade as a strategy for enhancing host defences against bacterial pathogens. Philos Trans R Soc Lond B Biol Sci. 2007; 362(1483):1213-22. PMC: 2435584. DOI: 10.1098/rstb.2007.2046. View

Manefield M, de Nys R, Naresh K, Roger R, Givskov M, Peter S . Evidence that halogenated furanones from Delisea pulchra inhibit acylated homoserine lactone (AHL)-mediated gene expression by displacing the AHL signal from its receptor protein. Microbiology (Reading). 1999; 145 ( Pt 2):283-291. DOI: 10.1099/13500872-145-2-283. View

Bratu S, Gupta J, Quale J . Expression of the las and rhl quorum-sensing systems in clinical isolates of Pseudomonas aeruginosa does not correlate with efflux pump expression or antimicrobial resistance. J Antimicrob Chemother. 2006; 58(6):1250-3. DOI: 10.1093/jac/dkl407. View

10.

Choo J, Rukayadi Y, Hwang J . Inhibition of bacterial quorum sensing by vanilla extract. Lett Appl Microbiol. 2006; 42(6):637-41. DOI: 10.1111/j.1472-765X.2006.01928.x. View

11.

Teasdale M, Liu J, Wallace J, Akhlaghi F, Rowley D . Secondary metabolites produced by the marine bacterium Halobacillus salinus that inhibit quorum sensing-controlled phenotypes in gram-negative bacteria. Appl Environ Microbiol. 2008; 75(3):567-72. PMC: 2632121. DOI: 10.1128/AEM.00632-08. View

12.

Kobayashi H . Airway biofilms: implications for pathogenesis and therapy of respiratory tract infections. Treat Respir Med. 2005; 4(4):241-53. DOI: 10.2165/00151829-200504040-00003. View

13.

Whitehead N, Barnard A, Slater H, Simpson N, Salmond G . Quorum-sensing in Gram-negative bacteria. FEMS Microbiol Rev. 2001; 25(4):365-404. DOI: 10.1111/j.1574-6976.2001.tb00583.x. View

14.

Frederiksen B, Koch C, Hoiby N . Antibiotic treatment of initial colonization with Pseudomonas aeruginosa postpones chronic infection and prevents deterioration of pulmonary function in cystic fibrosis. Pediatr Pulmonol. 1997; 23(5):330-5. DOI: 10.1002/(sici)1099-0496(199705)23:5<330::aid-ppul4>3.0.co;2-o. View

15.

Ohman D, Cryz S, Iglewski B . Isolation and characterization of Pseudomonas aeruginosa PAO mutant that produces altered elastase. J Bacteriol. 1980; 142(3):836-42. PMC: 294108. DOI: 10.1128/jb.142.3.836-842.1980. View

16.

Pearson J, Feldman M, Iglewski B, Prince A . Pseudomonas aeruginosa cell-to-cell signaling is required for virulence in a model of acute pulmonary infection. Infect Immun. 2000; 68(7):4331-4. PMC: 101761. DOI: 10.1128/IAI.68.7.4331-4334.2000. View

17.

Brint J, Ohman D . Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of RhlR-RhlI, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family. J Bacteriol. 1995; 177(24):7155-63. PMC: 177595. DOI: 10.1128/jb.177.24.7155-7163.1995. View

18.

OToole G, Kolter R . Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Mol Microbiol. 1998; 28(3):449-61. DOI: 10.1046/j.1365-2958.1998.00797.x. View

19.

Kessler E, Israel M, Landshman N, Chechick A, Blumberg S . In vitro inhibition of Pseudomonas aeruginosa elastase by metal-chelating peptide derivatives. Infect Immun. 1982; 38(2):716-23. PMC: 347797. DOI: 10.1128/iai.38.2.716-723.1982. View

20.

Dong Y, Gusti A, Zhang Q, Xu J, Zhang L . Identification of quorum-quenching N-acyl homoserine lactonases from Bacillus species. Appl Environ Microbiol. 2002; 68(4):1754-9. PMC: 123891. DOI: 10.1128/AEM.68.4.1754-1759.2002. View