Characterization of Three Monoclonal Antibodies That Recognize the Interferon Alpha 2 Receptor

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1990 Sep 1

PMID 2144902

Citations 6

Authors

O R Colamonici

F DAlessandro

M O Diaz

S A Gregory

L M Neckers

R Nordan

Affiliations

Soon will be listed here.

Abstract

The interferon system plays an important role in the control of viral infections and cell proliferation. These effects are mediated through the interaction of interferons with specific cell surface receptors. We report here the development of monoclonal antibodies against one of the subunits of the interferon alpha receptor. These antibodies detect a 110-kDa protein in surface-labeled cells and in Western blots, and 130- and 210-kDa bands after crosslinking to iodinated interferon alpha 2. No other subunits are disulfide-linked to the 130-kDa subunit or are coprecipitated by these antibodies. Analysis by two-dimensional gel electrophoresis revealed that the pI of this subunit is 3.5-5.0. We suggest that the protein recognized by these monoclonal antibodies be named the alpha subunit of the interferon alpha receptor.

Citing Articles

Molecular cloning and sequencing analysis of the interferon receptor (IFNAR-1) from Columba livia.

Li C, Chang W Cent Eur J Immunol. 2015; 39(2):159-64.

PMID: 26155117 PMC: 4440025. DOI: 10.5114/ceji.2014.43715.

A protein-arginine methyltransferase binds to the intracytoplasmic domain of the IFNAR1 chain in the type I interferon receptor.

Abramovich C, Yakobson B, Chebath J, Revel M EMBO J. 1997; 16(2):260-6.

PMID: 9029147 PMC: 1169633. DOI: 10.1093/emboj/16.2.260.

Differential tyrosine phosphorylation of the IFNAR chain of the type I interferon receptor and of an associated surface protein in response to IFN-alpha and IFN-beta.

Abramovich C, Shulman L, Ratovitski E, Harroch S, Tovey M, Eid P EMBO J. 1994; 13(24):5871-7.

PMID: 7813427 PMC: 395562. DOI: 10.1002/j.1460-2075.1994.tb06932.x.

Direct binding to and tyrosine phosphorylation of the alpha subunit of the type I interferon receptor by p135tyk2 tyrosine kinase.

Colamonici O, Yan H, Domanski P, Handa R, Smalley D, Mullersman J Mol Cell Biol. 1994; 14(12):8133-42.

PMID: 7526154 PMC: 359352. DOI: 10.1128/mcb.14.12.8133-8142.1994.

Expression and signaling specificity of the IFNAR chain of the type I interferon receptor complex.

Constantinescu S, Croze E, Murti A, Wang C, Basu L, Hollander D Proc Natl Acad Sci U S A. 1995; 92(23):10487-91.

PMID: 7479825 PMC: 40636. DOI: 10.1073/pnas.92.23.10487.

References

OFarrell P, Goodman H, Ofarrell P . High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977; 12(4):1133-41. DOI: 10.1016/0092-8674(77)90176-3. View

Aguet M . High-affinity binding of 125I-labelled mouse interferon to a specific cell surface receptor. Nature. 1980; 284(5755):459-61. DOI: 10.1038/284459a0. View

Mogensen K, Bandu M, Vignaux F, Aguet M, Gressner I . Binding of 125I-labelled human alpha interferon to human lymphoid cells. Int J Cancer. 1981; 28(5):575-82. DOI: 10.1002/ijc.2910280508. View

BRANCA A, Baglioni C . Evidence that types I and II interferons have different receptors. Nature. 1981; 294(5843):768-70. DOI: 10.1038/294768a0. View

Joshi A, Sarkar F, Gupta S . Interferon receptors. Cross-linking of human leukocyte interferon alpha-2 to its receptor on human cells. J Biol Chem. 1982; 257(23):13884-7. View

Faltynek C, BRANCA A, McCandless S, Baglioni C . Characterization of an interferon receptor on human lymphoblastoid cells. Proc Natl Acad Sci U S A. 1983; 80(11):3269-73. PMC: 394022. DOI: 10.1073/pnas.80.11.3269. View

Quesada J, Reuben J, Manning J, Hersh E, Gutterman J . Alpha interferon for induction of remission in hairy-cell leukemia. N Engl J Med. 1984; 310(1):15-8. DOI: 10.1056/NEJM198401053100104. View

Zoon K, Arnheiter H . Studies of the interferon receptors. Pharmacol Ther. 1984; 24(2):259-78. DOI: 10.1016/0163-7258(84)90037-8. View

Thompson M, Zhang Z, Fournier A, Tan Y . Characterization of human beta-interferon-binding sites on human cells. J Biol Chem. 1985; 260(1):563-7. View

10.

Shibata T, Shimada Y, Shimoyama M . Time-dependent cytotoxic action of human recombinant alpha-interferon (Ro22-8181) in vitro and the sensitivity of various cultured leukemia and lymphoma cell lines to it. Jpn J Clin Oncol. 1985; 15(1):67-75. View

11.

Clemens M, McNurlan M . Regulation of cell proliferation and differentiation by interferons. Biochem J. 1985; 226(2):345-60. PMC: 1144719. DOI: 10.1042/bj2260345. View

12.

Billard C, Sigaux F, Castaigne S, Valensi F, Flandrin G, Degos L . Treatment of hairy cell leukemia with recombinant alpha interferon: II. In vivo down-regulation of alpha interferon receptors on tumor cells. Blood. 1986; 67(3):821-6. View

13.

Gazdar A, Oie H, Kirsch I, Hollis G . Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. Blood. 1986; 67(6):1542-9. View

14.

Hannigan G, Lau A, Williams B . Differential human interferon alpha receptor expression on proliferating and non-proliferating cells. Eur J Biochem. 1986; 157(1):187-93. DOI: 10.1111/j.1432-1033.1986.tb09655.x. View

15.

Rubinstein M, Orchansky P . The interferon receptors. CRC Crit Rev Biochem. 1986; 21(3):249-75. DOI: 10.3109/10409238609113613. View

16.

Golomb H . The treatment of hairy cell leukemia. Blood. 1987; 69(4):979-83. View

17.

Talpaz M, Kantarjian H, McCredie K, Keating M, Trujillo J, Gutterman J . Clinical investigation of human alpha interferon in chronic myelogenous leukemia. Blood. 1987; 69(5):1280-8. View

18.

Pestka S, Langer J, Zoon K, Samuel C . Interferons and their actions. Annu Rev Biochem. 1987; 56:727-77. DOI: 10.1146/annurev.bi.56.070187.003455. View

19.

Quesada J, Gutterman J . Interferons in the treatment of human neoplasms. J Interferon Res. 1987; 7(5):575-81. DOI: 10.1089/jir.1987.7.575. View

20.

Talpaz M, Kantarjian H, Kurzrock R, Gutterman J . Therapy of chronic myelogenous leukemia: chemotherapy and interferons. Semin Hematol. 1988; 25(1):62-73. View